Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

被引：3

作者：

Liu, Zhigao ^{[1
]}

Korpelainen, Helena ^{[2
]}

机构：

[1] Zhejiang A&F Univ, Coll Landscape Architecture, Linan 311300, Zhejiang, Peoples R China

[2] Univ Helsinki, Dept Agr Sci, Viikki Plant Sci Ctr, POB 27, FIN-00014 Helsinki, Finland

来源：

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY | 2018年 / 59卷 / 06期

关键词：

Genetic markers; Genetic variation; Molecular identification; Ornamental plants; genetic relationships; DNA; RANUNCULACEAE; PHYLOGENY; GENOMES; CLASSIFICATION; FREQUENCY; REPEATS; RATES;

D O I：

10.1007/s13580-018-0090-3

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.

引用

页码：889 / 897

页数：9

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