Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

被引：3
|
作者：
Liu, Zhigao [1 ]
Korpelainen, Helena [2 ]
机构：
[1] Zhejiang A&F Univ, Coll Landscape Architecture, Linan 311300, Zhejiang, Peoples R China
[2] Univ Helsinki, Dept Agr Sci, Viikki Plant Sci Ctr, POB 27, FIN-00014 Helsinki, Finland
来源：
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY | 2018年 / 59卷 / 06期
关键词：
Genetic markers; Genetic variation; Molecular identification; Ornamental plants; genetic relationships; DNA; RANUNCULACEAE; PHYLOGENY; GENOMES; CLASSIFICATION; FREQUENCY; REPEATS; RATES;
D O I：
10.1007/s13580-018-0090-3
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.
引用
收藏
页码：889 / 897
页数：9
相关论文
共 50 条
  • [21] Production and molecular characterization of new citrus hybrids using somatic hybridization coupled with nuclear and chloroplastic microsatellite markers
    Chetto, O.
    Benyahia, H.
    Barantin, P.
    Ollitrault, P.
    Dambier, D.
    PROCEEDINGS OF THE IV INTERNATIONAL SYMPOSIUM ON CITRUS BIOTECHNOLOGY, 2019, 1230 : 15 - 22
  • [22] Population genetic structure and demographic history of Medicago ruthenica (Fabaceae) on the Qinghai-Tibetan Plateau based on nuclear ITS and chloroplast markers
    Wu, Xiaopei
    Liu, Demei
    Gulzar, Khan
    Shen, Yingfang
    Wang, Haiqing
    BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2016, 69 : 204 - 212
  • [23] Whole chloroplast genome sequences of the Japanese hemlocks, Tsuga diversifolia and T-sieboldii, and development of chloroplast microsatellite markers applicable to East Asian Tsuga
    Liu, L. -X.
    Li, P.
    Zhang, H. -W.
    Worth, J. R. P.
    JOURNAL OF FOREST RESEARCH, 2018, 23 (05) : 318 - 323
  • [24] Development of chloroplast-specific microsatellite markers for molecular characterization of alloplasmic lines and phylogenetic analysis in wheat
    Tomar, Ram Sewak S.
    Deshmukh, Rupesh K.
    Naik, Bhojaraja K.
    Tomar, Shiv Mangal S.
    Vinod
    PLANT BREEDING, 2014, 133 (01) : 12 - 18
  • [25] Phylogenetic relationships among Artemisia species based on nuclear ITS and chloroplast psbA-trnH DNA markers
    Ahmad Razban Haghighi
    Ali Osman Belduz
    Mohammad Moghaddam Vahed
    Kamil Coskuncelebi
    Salih Terzioglu
    Biologia, 2014, 69 : 834 - 839
  • [26] Molecular phylogeny of Cotoneaster (Rosaceae) inferred from nuclear ITS and multiple chloroplast sequences
    Li, Feifei
    Fan, Qiang
    Li, Qingyan
    Chen, Sufang
    Guo, Wei
    Cui, Dafang
    Liao, Wenbo
    PLANT SYSTEMATICS AND EVOLUTION, 2014, 300 (06) : 1533 - 1546
  • [27] Phylogenetic inferences in Prunus (Rosaceae) using chloroplast ndhF and nuclear ribosomal ITS sequences
    Jun Wen
    Berggren, Scott T.
    Chung-Hee Lee
    Ickert-Bond, Stefanie
    Ting-Shuang Yi
    Ki-Oug Yoo
    Lei Xie
    Shaw, Joey
    Potter, Dan
    JOURNAL OF SYSTEMATICS AND EVOLUTION, 2008, 46 (03) : 322 - 332
  • [28] Phylogenetic analysis of Asian Symplocos (Symplocaceae) based on nuclear and chloroplast DNA sequences
    Soejima, A
    Nagamasu, H
    JOURNAL OF PLANT RESEARCH, 2004, 117 (03) : 199 - 207
  • [29] Phylogenetic Analysis Based on Chloroplast and Nuclear DNA Sequences and a Revised Infrageneric Classification
    Watanabe, Seita T.
    Hayashi, Kazuhiko
    Arakawa, Katsuro
    Fuse, Shizuka
    Nagamasu, Hidetoshi
    Ikeda, Hiroshi
    Kuyama, Atsushi
    Suksathan, Piyakaset
    Poopath, Manop
    Pooma, Rachun
    Yang, Yong-Ping
    Tamura, Minoru N.
    ACTA PHYTOTAXONOMICA ET GEOBOTANICA, 2021, 72 (03) : 179 - 204
  • [30] Phylogenetic analysis of Asian Symplocos (Symplocaceae) based on nuclear and chloroplast DNA sequences
    Akiko Soejima
    Hidetoshi Nagamasu
    Journal of Plant Research, 2004, 117 : 199 - 207
12345