Intracellular Reactive Oxygen Species Production by Polymorphonuclear Leukocytes in Bovine Leukemia Virus-Infected Dairy Cows

被引：11

作者：

de Souza, Fernando Nogueira ^{[1
]}

Blagitz, Maiara Garcia ^{[1
]}

Latorre, Andreia Oliveira ^{[2
]}

Ramos Sanchez, Eduardo Milton ^{[3
]}

Batista, Camila Freitas ^{[1
]}

Weigel, Rebeca Alves ^{[1
]}

Renno, Francisco Palma ^{[4
]}

Araripe Sucupira, Maria Claudia ^{[1
]}

Melville Paiva Della Libera, Alice Maria ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Clin Sci, Fac Vet Med & Anim Sci, BR-05508270 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Pathol, Fac Vet Med & Anim Sci, BR-05508270 Sao Paulo, Brazil

[3] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo, Brazil

[4] Univ Sao Paulo, Dept Nutr & Anim Prod, Fac Vet Med & Anim Sci, BR-13635900 Pirassununga, Brazil

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2012年 / 74卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

deltaretrovirus; neutrophils; oxidative burst; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; IMMUNE-RESPONSE; BLV INFECTION; CATTLE; PHAGOCYTOSIS; TUBERCULOSIS; EXPRESSION; MASTITIS; SEQUENCE;

D O I：

10.1292/jvms.11-0246

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by now cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. caucus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.

引用

页码：221 / 225

页数：5

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