Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor

被引：84

作者：

Gao, Rongke ^{[1
]}

Ko, Juhui ^{[1
]}

Cha, Kiweon ^{[2
]}

Jeon, Jun Ho ^{[2
]}

Rhie, Gi-eun ^{[2
]}

Choi, Jonghoon ^{[1
]}

deMello, Andrew J. ^{[3
]}

Choo, Jaebum ^{[1
]}

机构：

[1] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea

[2] NIH, Div High Risk Pathogen Res, Ctr Infect Dis, Cheongju 363951, South Korea

[3] ETH, Inst Chem & Bioengn, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 72卷

基金：

新加坡国家研究基金会;

关键词：

Anthrax; Surface-enhanced Raman scattering; Microfluidics; Solenoid; Poly-gamma-D-glutamic acid; ENHANCED RAMAN-SCATTERING; D-GLUTAMIC ACID; BACILLUS-ANTHRACIS; COMPETITIVE IMMUNOASSAY; CANCER MARKERS; CELL ENTRY; CAPSULE; SYSTEMS; EDEMA;

D O I：

10.1016/j.bios.2015.05.005

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report the application of a fully automated surface-enhanced Raman scattering (SERS)-based solenoid-embedded microfluidic device to the quantitative and sensitive detection of anthrax biomarker poly-gamma-D-glutamic acid (PGA) in solution. Analysis is based on the competitive reaction between PGA and PGA-conjugated gold nanoparticles with anti-PGA-immobilized magnetic beads within a microfluidic environment. Magnetic immunocomplexes are trapped by yoke-type solenoids embedded within the device, and their SERS signals were directly measured and analyzed. To improve the acccuracy of measurement process, external standard values for PGA-free serum were also measured through use of a control channel. This additional measurement greatly improves the reliability of the assay by minimizing the influence of extraneous experimental variables. The limit of detection (LOD) of PGA in serum, determined by our SERS-based microfluidic sensor, is estimated to be 100 pg/mL. We believe that the defined method represents a valuable analytical tool for the detection of anthrax-related aqueous samples. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：230 / 236

页数：7

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