Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes

被引：194

作者：

Rodriguez, Marta ^{[1
,2
]}

Bajo-Santos, Cristina ^{[3
]}

Hessvik, Nina P. ^{[1
,2
]}

Lorenz, Susanne ^{[4
]}

Fromm, Bastian ^{[4
]}

Berge, Viktor ^{[5
]}

Sandvig, Kirsten ^{[1
,2
,6
]}

Line, Aija ^{[3
]}

Llorente, Alicia ^{[1
,2
]}

机构：

[1] Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Norwegian Radium Hosp, N-0379 Oslo, Norway

[2] Univ Oslo, Ctr Canc Biomed, Fac Med, N-0379 Oslo, Norway

[3] Latvian Biomed Res & Study Ctr, LV-1067 Riga, Latvia

[4] Oslo Univ Hosp, Norwegian Radium Hosp, Inst Canc Res, Dept Tumor Biol, N-0379 Oslo, Norway

[5] Oslo Univ Hosp, Dept Urol, N-0586 Oslo, Norway

[6] Univ Oslo, Dept Biosci, N-0316 Oslo, Norway

来源：

MOLECULAR CANCER | 2017年 / 16卷

关键词：

Biomarkers; Exosomes; Extracellular vesicles; microRNAs; Microvesicles; noncodingRNA; Next-generation sequencing; Prostate cancer; Small RNA; Urine; MICRORNA EXPRESSION SIGNATURE; EXTRACELLULAR VESICLES; LIQUID BIOPSY; TUMORS;

D O I：

10.1186/s12943-017-0726-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to identify microRNAs in urinary exosomes that are differently expressed in prostate cancer patients and healthy donors. For this purpose, RNA was extracted from urinary exosomes from 20 prostate cancer patients and 9 healthy males and the microRNAs were analyzed by next generation sequencing. Interestingly, 5 microRNAs -miR-196a-5p, miR-34a-5p, miR-143-3p, miR-501-3p and miR-92a-1-5p -were significantly downregulated in exosomes from prostate cancer patients. Furthermore, RT-qPCR analysis of an independent cohort of 28 prostate cancer patients and 19 healthy males confirmed that miR-196a-5p and miR-501-3p were downregulated in prostate cancer samples. These results suggest that specific microRNAs in urinary exosomes might serve as non-invasive biomarkers for prostate cancer. In particular, miR-196a-5p and miR-501-3p are promising biomarkers that need to be further studied in large patient cohorts.

引用

页数：6

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