An assessment of methods for aligning two-dimensional microscope sections to create image volumes

被引：13

作者：

Beare, R. ^{[1
]}

Richards, K. ^{[2
]}

Murphy, S. ^{[2
]}

Petrou, S. ^{[2
]}

Reutens, D. ^{[1
]}

机构：

[1] Monash Univ, Fac Med Nursing & Hlth Sci, Clayton, Vic 3168, Australia

[2] Univ Melbourne, Howard Florey Inst Expt Physiol & Med, Parkville, Vic 3010, Australia

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2008年 / 170卷 / 02期

关键词：

volume reconstruction; tissue section alignment; comparison of methods;

D O I：

10.1016/j.jneumeth.2008.01.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study assessed five different methods for aligning microscope images of Nissl-stained sections of mouse brain to form three-dimensional image volumes. Methods exploiting both image content and information from un-sectioned tissue were investigated. The accuracy of reconstruction was estimated using fiducials with known physical properties, demonstrating that methods exploiting tissue content produced distorted image volumes while a method using artificial fiducials produced the most accurate and unbiased alignment. Methodological issues relating to methods of volume reconstruction are discussed and it is recommended that methods using information from un-sectioned tissue be used wherever possible. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：332 / 344

页数：13

共 20 条

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