Molecular cloning and genomic analysis of TsNTxp:: an immunogenic protein from Tityus serrulatus scorpion venom

被引：17
作者：
Guatimosim, SC
Prado, VF
Diniz, CR
Chávez-Olórtegui, C
Kalapothakis, E [1 ]
机构：
[1] Univ Fed Minas Gerais, ICB, Dept Farmacol, Div Biol Celular, BR-31279901 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, ICB, Dept Bioquim Imunol, BR-31279901 Belo Horizonte, MG, Brazil
[3] Fdn Ezequiel Dias, Belo Horizonte, MG, Brazil
[4] Univ Fed Parana, Setor Ciencias Biol, Dept Patol Basica, Curitiba, Parana, Brazil
来源：
TOXICON | 1999年 / 37卷 / 03期
关键词：
toxin; ion channel; serotherapy; cDNA; genome sequencing; PCR;
D O I：
10.1016/S0041-0101(98)00187-1
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A non-toxic protein (TsNTxP) from Tityus serrulatus scorpion venom has been shown to be an efficient immunogen and anti-TsNTxP antibodies recognize and neutralize the effect of Tityus serrulatus venom [Chavez-Olortegui et al., 1997. Toxicon 35, 213 221]. With the purpose of studying the organization of the gene that code for this protein, we have isolated a full length cDNA clone for TsNTxP from a cDNA expression library using anti-TsNTxP antibodies. The nucleotide sequence of the gene that encodes TsNTxP was also obtained and it reveals the presence of an intron within the signal peptide sequence. The TsNTxP gene showed high degree of similarity with genes encoding toxins from scorpions of the genus Tityus. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：507 / 517
页数：11
相关论文
共 32 条
[1]   Toxic peptides and genes encoding toxin gamma of the Brazilian scorpions Tityus bahiensis and Tityus stigmurus [J].
Becerril, B ;
Corona, M ;
Coronas, FIV ;
Zamudio, F ;
CAlderonAranda, ES ;
Fletcher, PL ;
Martin, BM ;
Possani, LD .
BIOCHEMICAL JOURNAL, 1996, 313 :753-760
[2]   THE GENOMIC REGION ENCODING TOXIN-GAMMA FROM THE SCORPION TITYUS-SERRULATUS CONTAINS AN INTRON [J].
BECERRIL, B ;
CORONA, M ;
MEJIA, MC ;
MARTIN, BM ;
LUCAS, S ;
BOLIVAR, F ;
POSSANI, LD .
FEBS LETTERS, 1993, 335 (01) :6-8
[3]   SELECTIVE BLOCKAGE OF VOLTAGE-DEPENDENT K+ CHANNELS BY A NOVEL SCORPION TOXIN [J].
CARBONE, E ;
WANKE, E ;
PRESTIPINO, G ;
POSSANI, LD ;
MAELICKE, A .
NATURE, 1982, 296 (5852) :90-91
[4]   Neutralizing capacity of antibodies elicited by a non-toxic protein purified from the venom of the scorpion Tityus serrulatus [J].
ChavezOlortegui, C ;
Kalapothakis, E ;
Ferreira, AMBM ;
Ferreira, AP ;
Diniz, CR .
TOXICON, 1997, 35 (02) :213-221
[5]  
CHAVEZOLORTEGUI C, 1996, P 1 INT C I PAST PAR, P183
[6]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[7]   Cloning and characterization of the genomic region encoding toxin IV-5 from the scorpion Tityus serrulatus Lutz and Mello [J].
Corona, M ;
Zurita, M ;
Possani, LD ;
Becerril, B .
TOXICON, 1996, 34 (02) :251-256
[8]   2 TYPES OF SCORPION TOXIN RECEPTOR-SITES, ONE RELATED TO THE ACTIVATION, THE OTHER TO THE INACTIVATION OF THE ACTION-POTENTIAL SODIUM-CHANNEL [J].
COURAUD, F ;
JOVER, E ;
DUBOIS, JM ;
ROCHAT, H .
TOXICON, 1982, 20 (01) :9-16
[9]   PURIFICATION AND CHARACTERIZATION OF CHLOROTOXIN, A CHLORIDE CHANNEL LIGAND FROM THE VENOM OF THE SCORPION [J].
DEBIN, JA ;
MAGGIO, JE ;
STRICHARTZ, GR .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (02) :C361-C369
[10]  
DELIMA ME, 1995, J TOXICOL-TOXIN REV, V14, P457, DOI 10.3109/15569549509019474
1234