Epitope tagging

被引：141

作者：

Jarvik, JW ^{[1
]}

Telmer, CA ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Biol Sci, Pittsburgh, PA 15213 USA

来源：

ANNUAL REVIEW OF GENETICS | 1998年 / 32卷

关键词：

epitope tagging; protein fusion; gene fusion; antibodies; proteomics;

D O I：

10.1146/annurev.genet.32.1.601

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. This review discusses the major advantages and limitations of epitope tagging and describes a number of recent applications. Major areas of application include monitoring protein expression, localizing proteins at the cellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Recently the method has also found use in transgenic and gene therapy studies and in the emerging fields of functional genomics and proteomics.

引用

页码：601 / 618

页数：18

共 121 条

[1] REPORTER EPITOPES - A NOVEL-APPROACH TO EXAMINE TRANSMEMBRANE TOPOLOGY OF INTEGRAL MEMBRANE-PROTEINS APPLIED TO THE ALPHA-1 SUBUNIT OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR [J].

ANAND, R ;

BASON, L ;

SAEDI, MS ;

GERZANICH, V ;

PENG, X ;

LINDSTROM, J .

BIOCHEMISTRY, 1993, 32 (38) :9975-9984

[2]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[3]

ATKIN AL, 1995, MOL BIOL CELL, V6, P611

[4]

BENITO A, 1994, FEMS MICROBIOL LETT, V123, P107, DOI 10.1111/j.1574-6968.1994.tb07208.x

[5] The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity [J].

Berges, T ;

Guyonnet, D ;

Karst, F .

JOURNAL OF BACTERIOLOGY, 1997, 179 (15) :4664-4670

[6] Oligomeric structure of hepatic lipase, evidence from a novel epitope tag technique [J].

Berryman, DE ;

Mulero, JJ ;

Hughes, LB ;

Brasaemle, DL ;

Bensadoun, A .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1998, 1382 (02) :217-229

[7]

BORJIGIN J, 1994, J BIOL CHEM, V269, P14715

[8] EPITOPE-LABELED SOLUBLE HUMAN INTERLEUKIN-5 (IL-5) RECEPTORS - AFFINITY CROSS-LINK LABELING, IL-5 BINDING, AND BIOLOGICAL-ACTIVITY [J].

BROWN, PM ;

TAGARI, P ;

ROWAN, KR ;

YU, VL ;

ONEILL, GP ;

MIDDAUGH, RC ;

SANYAL, G ;

FORDHUTCHINSON, AW ;

NICHOLSON, DW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (49) :29236-29243

[9] Localization of cytoplasmic and extracellular domains of Na,K-ATPase by epitope tag insertion [J].

Canfield, VA ;

Norbeck, L ;

Levenson, R .

BIOCHEMISTRY, 1996, 35 (45) :14165-14172

[10] DISSECTION OF FUNCTIONAL DOMAINS OF ADENOVIRUS DNA-POLYMERASE BY LINKER-INSERTION MUTAGENESIS [J].

CHEN, M ;

HORWITZ, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6116-6120

← 1 2 3 4 5 6 7 8 9 10 →