DOTA-Amide Lanthanide Tag for Reliable Generation of Pseudocontact Shifts in Protein NMR Spectra

被引：92

作者：

Graham, Bim ^{[2
]}

Choy Theng Loh ^{[1
]}

Swarbrick, James David ^{[2
]}

Phuc Ung ^{[2
]}

Shin, James ^{[2
]}

Yagi, Hiromasa ^{[1
]}

Jia, Xinying ^{[1
]}

Chhabra, Sandeep ^{[2
]}

Barlow, Nicholas ^{[2
]}

Pintacuda, Guido ^{[3
]}

Huber, Thomas ^{[1
]}

Otting, Gottfried ^{[1
]}

机构：

[1] Australian Natl Univ, Res Sch Chem, Canberra, ACT 0200, Australia

[2] Monash Inst Pharmaceut Sci, Parkville, Vic 3052, Australia

[3] ENS Lyon, Ctr RMN Tres Hauts Champs, F-69100 Villeurbanne, France

来源：

BIOCONJUGATE CHEMISTRY | 2011年 / 22卷 / 10期

基金：

澳大利亚研究理事会;

关键词：

PARAMAGNETIC NMR; BINDING TAG; MAGNETIC-SUSCEPTIBILITY; LIGAND COMPLEXES; WATER EXCHANGE; SPECTROSCOPY; ACID; DISTANCE; DESIGN; SERIES;

D O I：

10.1021/bc200353c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural studies of proteins and protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy can be greatly enhanced by site-specific attachment of lanthanide ions to create paramagnetic centers. In particular, pseudocontact shifts (PCS) generated by paramagnetic lanthanides contain important and unique long-range structure information. Here, we present a high-affinity lanthanide binding tag that can be attached to single cysteine residues of proteins. The new tag has many advantageous features that are not available in this combination from previously published tags: (i) it binds lanthanide ions very tightly, minimizing the generation of nonspecific effects, (ii) it produces PCSs with high reliability as its bulkiness prevents complete motional averaging of PCSs, (iii) it can be attached to single cysteine residues, alleviating the need of detailed prior knowledge of the 3D structure of the target protein, and (iv) it does not display conformational exchange phenomena that would increase the number of signals in the NMR spectrum. The performance of the tag is demonstrated with the N-terminal domain of the E. coli arginine repressor and the A28C mutant of human ubiquitin. © 2011 American Chemical Society.

引用

页码：2118 / 2125

页数：8

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