Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana

被引：96
作者：
Liu, Yajie [1 ]
Jiang, Haifeng [2 ]
Zhao, Zhiguang [1 ]
An, Lizhe [1 ]
机构：
[1] Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Arid & Grassland Agroecol, Lanzhou 730000, Peoples R China
[2] Gansu Prov Peoples Hosp, Dept Radiol, Lanzhou 730000, Peoples R China
来源：
JOURNAL OF PLANT PHYSIOLOGY | 2011年 / 168卷 / 09期
基金：
国家高技术研究发展计划(863计划); 美国国家科学基金会;
关键词：
Abscisic acid; Antioxidant defense; Brassinosteroids; Chilling; Chorispora bungeana; CHICKPEA CICER-ARIETINUM; ANTIOXIDANT SYSTEMS; HYDROGEN-PEROXIDE; SUPEROXIDE-DISMUTASE; OXIDATIVE STRESS; MAIZE SEEDLINGS; COLD TOLERANCE; ACCUMULATION; GROWTH; HEAT;
D O I：
10.1016/j.jplph.2010.09.020
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The objective of this study was to investigate whether abscisic acid (ABA), a second messenger in chilling stress responses, is involved in brassinosteroids (BRs)-induced chilling tolerance in suspension cultured cells from Chorispora bungeana. The suspension cells were treated with 24-epibrassinolide (EBR), ABA, ABA biosynthesis inhibitor fluridone (Flu) and EBR in combination with Flu. Their effects on chilling tolerance, reactive oxygen species (ROS) levels and antioxidant defense system were analyzed. The results showed that EBR treatment markedly alleviated the decrease of cell viability and the increases of ion leakage and lipid peroxidation induced by chilling stress, suggesting that application of EBR could improve the chilling tolerance of C. bungeana suspension cultures. In addition, similar results were observed when exogenous ABA was applied. Treatment with Flu alone and in combination with EBR significantly suppressed cell viability and increased ion leakage and lipid peroxidation under low temperature conditions, indicating that the inhibition of ABA biosynthesis could decrease the chilling tolerance of C. bungeana suspension cultures and the EBR-enhanced chilling tolerance. Further analyses showed that EBR and ABA enhanced antioxidant defense and slowed down the accumulation of ROS caused by chilling. However, Flu application differentially blocked these protective effects of EBR. Moreover, EBR was able to mimic the effect of ABA by markedly increasing ABA content in the suspension cells under chilling conditions, whereas the EBR-induced ABA accumulation was inhibited by the addition of Flu. Taken together, these results demonstrate that EBR may confer chilling tolerance to C. bungeana suspension cultured cells by enhancing the antioxidant defense system, which is partially mediated by ABA, resulting in preventing the overproduction of ROS to alleviate oxidative injury induced by chilling. (C) 2011 Elsevier GmbH. All rights reserved.
引用
收藏
页码：853 / 862
页数：10
相关论文
共 49 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean (Vigna radiata L. Wilczek) [J].
Ali, B. ;
Hasan, S. A. ;
Hayat, S. ;
Hayat, Q. ;
Yadav, S. ;
Fariduddin, Q. ;
Ahmad, A. .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2008, 62 (02) :153-159
[3]   Brassinosteroid enhanced the level of abscisic acid in Chlorella vulgaris subjected to short-term heat stress [J].
Bajguz, Andrzej .
JOURNAL OF PLANT PHYSIOLOGY, 2009, 166 (08) :882-886
[4]   Effects of brassinosteroids on the plant responses to environmental stresses [J].
Bajguz, Andrzej ;
Hayat, Shamsul .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2009, 47 (01) :1-8
[5]  
Beauchamp C., 1971, ANAL BIOCHEM, V44, P276, DOI DOI 10.1016/0003-2697(71)90370-8
[6]   SUPEROXIDE-DISMUTASE AND STRESS TOLERANCE [J].
BOWLER, C ;
VANMONTAGU, M ;
INZE, D .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :83-116
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   ASSAY OF CATALASES AND PEROXIDASES [J].
CHANCE, B ;
MAEHLY, AC .
METHODS IN ENZYMOLOGY, 1955, 2 :764-775
[9]   Chilling-induced Ca2+ overload enhances production of active oxygen species in maize (Zea mays L.) cultured cells:: the effect of abscisic acid treatment [J].
Chen, WP ;
Li, PH .
PLANT CELL AND ENVIRONMENT, 2001, 24 (08) :791-800
[10]   Drought survival and recuperative ability of bentgrass species associated with changes in abscisic acid and cytokinin production [J].
DaCosta, Michelle ;
Huang, Bingru .
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2007, 132 (01) :60-66
12345