Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus

被引：8

作者：

Go, Hyeon-Jeong ^{[1
]}

Park, Byung-Joo ^{[1
]}

Ahn, Hee-Seop ^{[1
]}

Kim, Dong-Hwi ^{[1
]}

Kim, Da-Yoon ^{[1
]}

Kim, Jae-Hyeong ^{[1
]}

Lee, Joong-Bok ^{[1
]}

Park, Seung-Yong

Song, Chang-Seon ^{[1
]}

Lee, Sang-Won ^{[1
]}

Choi, Yang-Kyu ^{[2
]}

Choi, In-Soo ^{[1
]}

机构：

[1] Konkuk Univ, Dept Infect Dis, Coll Vet Med, 120 Neundong Ro, Seoul 05029, South Korea

[2] Konkuk Univ, Dept Lab Anim Med, Coll Vet Med, 120 Neundong Ro, Seoul 05029, South Korea

来源：

VACCINES | 2021年 / 9卷 / 11期

关键词：

Hepatitis E virus; virus-like particle; 239 amino acids; viremia; fecal viral shedding; liver fibrosis; baculovirus expression system; EXPRESSION; SEQUENCES; EFFICACY; SAFETY;

D O I：

10.3390/vaccines9111265

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367-605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive control, 100 mu g VLP-, and 200 mu g VLP-vaccinated groups for 10 weeks. The pigs in either of the vaccinated groups were administered the corresponding first and booster doses on weeks 0 and 2. At week 4, the positive control and two vaccinated groups were challenged with 10(6) HEV-3 genomic equivalent copies; viremia and fecal shedding of the virus were identified in pigs in the positive control and 100 mu g VLP-vaccinated pigs showed transient viremia and fecal viral shedding. However, no viruses were detected in the serum or fecal samples of the 200 mu g VLP-vaccinated pigs. The 100 and 200 mu g VLP-vaccinated pigs had significantly higher (p < 0.01) anti-HEV antibodies than the negative control pigs from weeks 6-10 with normal levels of liver enzymes. The 200 mu g VLP-vaccinated pigs showed statistically less liver tissue fibrosis (p < 0.05) than that of the positive control pigs. Thus, the novel baculovirus expression system-generated VLP vaccine dose-dependently protects against HEV-3 challenge and may be useful in other animal species, including humans.

引用

页数：12

共 33 条

[1] Molecular virology of hepatitis E virus [J].