Bioelectrocatalytic determination of nitrite ions based on polyaniline grafted nanodiamond

被引：56

作者：

Gopalan, A. I. ^{[1
,2
]}

Lee, K. -P. ^{[1
,2
]}

Komathi, S. ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Chem Educ, Taegu, South Korea

[2] Kyungpook Natl Univ, Dept Nanosci & Technol, Taegu, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 26卷 / 04期

关键词：

Nanodiamond; Polyaniline; Grafting; Gold; Cytochrome c; Nitrite; DIRECT ELECTRON-TRANSFER; CYTOCHROME-C; GOLD NANOPARTICLES; DIRECT ELECTROCHEMISTRY; GLUCOSE BIOSENSOR; REDUCTASE; TEMPERATURE; NITRATE; FABRICATION; PROTEINS;

D O I：

10.1016/j.bios.2010.08.042

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Polyaniline chains were grafted onto nanodiamond (PANI-g-ND) through electrochemical polymerization of aniline in the presence of amine functionalized ND. A robust and effective composite film comprising PANI-g-ND/gold particles was subsequently prepared. Cytochrome c was successfully immobilized onto PANI-g-ND/Au film. Field emission scanning electron microscope (FESEM) image of PANI-g-ND/Au reveals the presence of fibrous PANI embedded into ND galleries with uniformly distributed Au clusters (similar to 1 mu m). Direct electrochemistry and electrocatalysis of cyt c were investigated. PANI-g-ND/Au film showed an obvious direct electron transfer between cyt c and the underlying electrode. Cyclic voltammograms revealed that PANI-g-ND/Au/cyt c exhibited an excellent electrocatalysis towards the detection of nitrite ions. Differential pulse voltammetry of PANI-g-ND/Au/cyt c revealed a wide linear concentration range (0.5 mu M-3 mM) for current responses, sensitivity (88.2 mu A/mM) and low detection limit (0.16 mu M) towards the electrochemical detection of nitrite ions. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1638 / 1643

页数：6

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