Ectoenzymes in Prorocentrum minimum

被引：42

作者：

Dyhrman, S ^{[1
]}

机构：

[1] Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA

来源：

HARMFUL ALGAE | 2005年 / 4卷 / 03期

关键词：

alkaline phosphatase (AP); ectoenzyme; leucine aminopeptidase (LAP); Prorocentrum minimum;

D O I：

10.1016/j.hal.2004.08.011

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Ectoenzymes, or enzymes associated with the cell-surface or periplasmic space, play an important role in organic matter cycling by rendering certain forms of dissolved organic matter bioavailable. Ectoenzyme activities may thereby help meet the nutritional demands of harmful algae such as Prorocentrum minimum. The activities of two ectoenzymes; leucine aminopeptidase and alkaline phosphatase, have been studied in axenic cultures of P. minimum. Leucine aminopeptidase releases nonpolar amino acids such as leucine from the N-terminus of polypeptides, whereas alkaline phosphatase is an enzyme that is able to hydrolyze phosphate from phosphomonoesters. P. minimum alkaline phosphatase is the better studied of the two ectoenzymes and its characteristics are reviewed herein. Future research on P. minimum physiology will benefit from a growing suite of tools available for assessing the activity of alkaline phosphatase and other ectoenzymes in field populations and ultimately the work done with P. minimum will be useful for studies of other harmful species. (C) 2004 Elsevier B.V. All fights reserved.

引用

页码：619 / 627

页数：9

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