Acute oral toxicity evaluation of extracts of Hydrocotyle sibthorpioides in wister albino rats as per OECD 425 TG

Hydrocotyle sibthorpioides (Family: Apiaceae) is a medicinal plant and is been a part of traditional cuisine. Hence, an acute toxicity study was performed to confirm its safety profile. Female rats (n = 5) were randomly divided into 5 groups. One group served as the control and the other groups were been administered with the petroleum ether, chloroform, methanolic and aqueous extract of Hydrocotyle sibthorpioides at 2000 mg/kg body weight. Blood was collected at certain intervals to measure the haematological and biochemical parameters. The vital organs were isolated and preserved for histopathological examinations. The result suggested that the LD50 of all the extracts are greater than 2000 mg/kg body weight. There was no significant change in body weight, Behaviour, Renal Function test, Liver function test and Lipid profile. In haematological evaluation, there was an increase in RBC count and haemoglobin production post administration of methanolic and aqueous extract. The study suggested that the LD50 of the plant is greater than 2000 mg/kg body weight of rats in all the extracts.