Nanofluidics for Simultaneous Size and Charge Profiling of Extracellular Vesicles

被引:17
作者
Hosseini, Imman I. [1 ]
Liu, Zezhou [2 ]
Capaldi, Xavier [2 ]
AbdelFatah, Tamer [1 ]
Montermini, Laura [3 ]
Rak, Janusz [4 ]
Reisner, Walter [2 ]
Mahshid, Sara [1 ]
机构
[1] McGill Univ, Dept Bioengn, Montreal, PQ H3A 2B4, Canada
[2] McGill Univ, Dept Phys, Montreal, PQ H3A 2T8, Canada
[3] McGill Univ Hlth Ctr, Res Inst, Montreal, PQ H4A 3J1, Canada
[4] McGill Univ, Res Inst, McGill Univ Hlth Ctr, Dept Pediat, Montreal, PQ H4A 3J1, Canada
基金
加拿大自然科学与工程研究理事会; 加拿大创新基金会;
关键词
Extracellular vesicles; tunable confinement; nanofluidics; EXOSOMES; MICROVESICLES; OBJECTS; BLOOD; LIGHT;
D O I
10.1021/acs.nanolett.0c02558
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Extracellular vesicles (EVs) are cell-derived membrane structures that circulate in body fluids and show considerable potential for noninvasive diagnosis. EVs possess surface chemistries and encapsulated molecular cargo that reflect the physiological state of cells from which they originate, including the presence of disease. In order to fully harness the diagnostic potential of EVs, there is a critical need for technologies that can profile large EV populations without sacrificing single EV level detail by averaging over multiple EVs. Here we use a nanofluidic device with tunable confinement to trap EVs in a free-energy landscape that modulates vesicle dynamics in a manner dependent on EV size and charge. As proof-of-principle, we perform size and charge profiling of a population of EVs extracted from human glioblastoma astrocytoma (U373) and normal human astrocytoma (NHA) cell lines.
引用
收藏
页码:4895 / 4902
页数:8
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