Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay

被引：69
作者：
Naserke, HE
Dozio, N
Ziegler, AG
Bonifacio, E
机构：
[1] San Raffaele Sci Inst, Dept Med 1, I-20132 Milan, Italy
[2] Diabet Res Inst, Munich, Germany
[3] Acad Hosp Schwabing, Munich, Germany
来源：
DIABETOLOGIA | 1998年 / 41卷 / 06期
关键词：
insulin autoantibodies; radiobinding assay; prediction; insulin-dependent diabetes mellitus;
D O I：
10.1007/s001250050968
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 mu l of serum was compared with that in a conventional radiobinding assay which uses 600 mu l of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects, and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95%) of 239 samples tested were concordant, and IAA levels correlated well (r(2) = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative; and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69% and 98% for the micro radiobinding assay and 72% and 98% for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children.
引用
收藏
页码：681 / 683
页数：3
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