A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples

被引：33
作者：
Fu, Xiaoxiang [1 ]
Wang, Xiaohan [1 ]
Cui, Yongliang [1 ]
Wang, Ali [1 ]
Lai, Daowan [1 ]
Liu, Yang [2 ]
Li, Qing X. [3 ]
Wang, Baomin [1 ]
Zhou, Ligang [1 ]
机构：
[1] China Agr Univ, Coll Agron & Biotechnol, Being 100193, Peoples R China
[2] Chinese Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Beijing 100193, Peoples R China
[3] Univ Hawaii, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA
来源：
FOOD CHEMISTRY | 2015年 / 181卷
基金：
中国国家自然科学基金;
关键词：
Ustiloxin A; icELISA; Mycotoxin; Phytotoxin; Rice false smut; Villosiclava virens; ANTIMITOTIC CYCLIC-PEPTIDES; USTILAGINOIDEA-VIRENS; BRAIN TUBULIN; PANICLES; INOCULATION;
D O I：
10.1016/j.foodchem.2015.02.068
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Ustiloxin A, a cyclopeptide mycotoxin, was isolated from the pathogenic fungus Villosiclava virens that causes rice false smut, a worldwide devastating rice disease. A monoclonal antibody (mAb) 2D3G5 was generated with ustiloxin A-bovine serum albumin conjugate. A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. It possessed a median inhibition concentration (IC50) of 13.8 ng/mL and a working range of 2.8-72 ng/mL. The mAb 2D3G5 recognized ustiloxin B with the cross-reactivity as 4%. The average recoveries of ustiloxin A from rice false smut balls and peeled rice samples ranged from 92% to 117% and from 92% to 107%, respectively. Comparison of the concentrations of ustiloxin A in rice false smut balls detected by both icELISA and high performance liquid chromatography-photodiode array detection indicated that the developed icELISA was suitable for detection of ustiloxin A in rice food and feed samples. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：140 / 145
页数：6
相关论文
共 28 条
[1]   Quantification of the rice false smut pathogen Ustilaginoidea virens from soil in Japan using real-time PCR [J].
Ashizawa, Taketo ;
Takahashi, Mami ;
Moriwaki, Jouji ;
Hirayae, Kazuyuki .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2010, 128 (02) :221-232
[2]   Generation of class-selective monoclonal antibodies against the penicillin group [J].
Cliquet, P ;
Cox, E ;
Van Dorpe, C ;
Schacht, E ;
Goddeeris, BM .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (07) :3349-3355
[3]   Development of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for analysing chlorantraniliprole residues [J].
Cui, Yongliang ;
Liu, Kechang ;
Xu, Chuan ;
Liu, Feng ;
Li, Qing X. ;
Liu, Shangzhong ;
Wang, Baomin .
FOOD CHEMISTRY, 2014, 143 :293-299
[4]  
Guo XiaoYi Guo XiaoYi, 2012, Journal of Agricultural Science and Technology A, V2, P1211
[5]   Infection processes of Ustilaginoidea virens during artificial inoculation of rice panicles [J].
Hu, Maolin ;
Luo, Laixin ;
Wang, Shu ;
Liu, Yongfeng ;
Li, Jianqiang .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2014, 139 (01) :67-77
[6]  
Ji Fang, 2012, Zhongguo Shuidao Kexue, V26, P246
[7]   USTILOXINS, ANTIMITOTIC CYCLIC-PEPTIDES FROM FALSE SMUT BALLS ON RICE PANICLES CAUSED BY USTILAGINOIDEA VIRENS [J].
KOISO, Y ;
LI, Y ;
IWASAKI, S ;
HANAOKA, K ;
KOBAYASHI, T ;
SONODA, R ;
FUJITA, Y ;
YAEGASHI, H ;
SATO, Z .
JOURNAL OF ANTIBIOTICS, 1994, 47 (07) :765-773
[8]   Isolation and structure of an antimitotic cyclic peptide, Ustiloxin F: Chemical interrelation with a homologous peptide, Ustiloxin B [J].
Koiso, Y ;
Morisaki, N ;
Yamashita, Y ;
Mitsui, Y ;
Shirai, R ;
Hashimoto, Y ;
Iwasaki, S .
JOURNAL OF ANTIBIOTICS, 1998, 51 (04) :418-422
[9]   USTILOXIN - A PHYTOTOXIN AND A MYCOTOXIN FROM FALSE SMUT BALLS ON RICE PANICLES [J].
KOISO, Y ;
NATORI, M ;
IWASAKI, S ;
SATO, S ;
SONODA, R ;
FUJITA, Y ;
YAEGASHI, H ;
SATO, Z .
TETRAHEDRON LETTERS, 1992, 33 (29) :4157-4160
[10]   USTILOXINS, NEW ANTIMITOTIC CYCLIC-PEPTIDES - INTERACTION WITH PORCINE BRAIN TUBULIN [J].
LI, Y ;
KOISO, Y ;
KOBAYASHI, H ;
HASHIMOTO, Y ;
IWASAKI, S .
BIOCHEMICAL PHARMACOLOGY, 1995, 49 (10) :1367-1372
123