Polymerization of the backbone of the pectic polysaccharide rhamnogalacturonan I

被引:22
作者
Amos, Robert A. [1 ,2 ]
Atmodjo, Melani A. [1 ,2 ]
Huang, Chin [1 ,2 ]
Gao, Zhongwei [2 ]
Venkat, Aarya [1 ]
Taujale, Rahil [1 ]
Kannan, Natarajan [1 ]
Moremen, Kelley W. [1 ,2 ]
Mohnen, Debra [1 ,2 ]
机构
[1] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
[2] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
基金
美国国家卫生研究院;
关键词
ARABIDOPSIS-THALIANA; IDENTIFICATION; BIOSYNTHESIS; ALIGNMENT; PROTEINS; MUCILAGE; ENZYMES; GROWTH;
D O I
10.1038/s41477-022-01270-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rhamnogalacturonan I (RG-I) is a major plant cell wall pectic polysaccharide defined by its repeating disaccharide backbone structure of [4)-alpha-d-GalA-(1,2)-alpha-l-Rha-(1,]. A family of RG-I:Rhamnosyltransferases (RRT) has previously been identified, but synthesis of the RG-I backbone has not been demonstrated in vitro because the identity of Rhamnogalacturonan I:Galaturonosyltransferase (RG-I:GalAT) was unknown. Here a putative glycosyltransferase, At1g28240/MUCI70, is shown to be an RG-I:GalAT. The name RGGAT1 is proposed to reflect the catalytic activity of this enzyme. When incubated together with the rhamnosyltransferase RRT4, the combined activities of RGGAT1 and RRT4 result in elongation of RG-I acceptors in vitro into a polymeric product. RGGAT1 is a member of a new GT family categorized as GT116, which does not group into existing GT-A clades and is phylogenetically distinct from the GALACTURONOSYLTRANSFERASE (GAUT) family of GalA transferases that synthesize the backbone of the pectin homogalacturonan. RGGAT1 has a predicted GT-A fold structure but employs a metal-independent catalytic mechanism that is rare among glycosyltransferases with this fold type. The identification of RGGAT1 and the 8-member Arabidopsis GT116 family provides a new avenue for studying the mechanism of RG-I synthesis and the function of RG-I in plants. The glycosyltransferase enzyme RGGAT1 is shown to catalyse the addition of galacturonic acid into rhamnogalacturonan I, the backbone of the plant cell wall, with implications for in vitro pectin synthesis.
引用
收藏
页码:1289 / +
页数:28
相关论文
共 50 条
  • [21] Rhamnogalacturonan-I-Type Polysaccharide Purified from Broccoli Exerts Anti-Metastatic Activities Via Innate Immune Cell Activation
    Kwak, Bong-Shin
    Hwang, Dahyun
    Lee, Sue Jung
    Choi, Hyuk-Jun
    Park, Ho-Young
    Shin, Kwang-Soon
    [J]. JOURNAL OF MEDICINAL FOOD, 2019, 22 (05) : 451 - 459
  • [22] Boron bridging of rhamnogalacturonan-II, monitored by gel electrophoresis, occurs during polysaccharide synthesis and secretion but not post-secretion
    Chormova, Dimitra
    Messenger, David J.
    Fry, Stephen C.
    [J]. PLANT JOURNAL, 2014, 77 (04) : 534 - 546
  • [23] A Highly Efficient Biocatalytic Conversion of Renewable Pectic Polysaccharide Biomass into Bioactive Oligogalacturonides
    Yang, Guojun
    Li, Shuguang
    Tan, Haidong
    Li, Kuikui
    Chen, Wei
    Yin, Heng
    [J]. ACS FOOD SCIENCE & TECHNOLOGY, 2021, 1 (03): : 338 - 346
  • [24] Rhamnogalacturonan I galactosyltransferase: Detection of enzyme activity and its hyperactivation
    Matsumoto, Naoki
    Takenaka, Yuto
    Wachananawat, Bussarin
    Kajiura, Hiroyuki
    Imai, Tomoya
    Ishimizu, Takeshi
    [J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2019, 142 : 173 - 178
  • [25] The roles and mechanisms of homogalacturonan and rhamnogalacturonan I pectins on the inhibition of cell migration
    Fan, Yuying
    Sun, Lin
    Yang, Siwen
    He, Congcong
    Tai, Guihua
    Zhou, Yifa
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 106 : 207 - 217
  • [26] Crystal structure of exo-rhamnogalacturonan lyase from Penicillium chrysogenum as a member of polysaccharide lyase family 26
    Kunishige, Yuika
    Iwai, Marin
    Nakazawa, Masami
    Ueda, Mitsuhiro
    Tada, Toshiji
    Nishimura, Shigenori
    Sakamoto, Tatsuji
    [J]. FEBS LETTERS, 2018, 592 (08) : 1378 - 1388
  • [27] Structural elucidation of a pectic polysaccharide from Fructus Mori and its bioactivity on intestinal bacteria strains
    Li, Saijuan
    Li, Meixia
    Yue, Han
    Zhou, Lishuang
    Huang, Lulin
    Du, Zhenyun
    Ding, Kan
    [J]. CARBOHYDRATE POLYMERS, 2018, 186 : 168 - 175
  • [28] Large-scale extraction of rhamnogalacturonan I from industrial potato waste
    Byg, Inge
    Diaz, Jerome
    Ogendal, Lars Holm
    Harholt, Jesper
    Jorgensen, Bodil
    Rolin, Claus
    Svava, Rikke
    Ulvskov, Peter
    [J]. FOOD CHEMISTRY, 2012, 131 (04) : 1207 - 1216
  • [29] Mutations in type II Golgi-localized proton pyrophosphatase AVP2;1/VHP2;1 affect pectic polysaccharide rhamnogalacturonan-II and alter root growth under low boron condition in Arabidopsis thaliana
    Onuh, Amarachukwu Faith
    Miwa, Kyoko
    [J]. FRONTIERS IN PLANT SCIENCE, 2023, 14
  • [30] Chemical structure and immunoinhibitory activity of a pectic polysaccharide containing glucuronic acid from the leaves of Diospyros kaki
    Duan, Jinyou
    Chen, Vincent L.
    Dong, Qun
    Ding, Kan
    Fang, Jinian
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2010, 46 (05) : 465 - 470