Involvement of prostaglandins in the down-regulation of allergic plasma leakage observed in rats undergoing pleural eosinophilia

1 Recent evidence has implicated eosinophils in the inhibition of allergen-induced rat pleurisy, but the mechanism of this negative modulation is not completely understood. This study was undertaken in order to define the potential role of prostaglandins in this phenomenon. 2 Wistar rats were actively sensitized by subcutaneous injection of a mixture of ovalbumin and Al(OH)(3) and challenged with an intrapleural (i.pl.) injection of ovalbumin (12 mu g/cavity) 14 days later. 3 Analysis of the pleural fluid effluent revealed a massive mast cell degranulation and plasma protein extravasation 4 h post-challenge. We confirmed that concurrently with selective pleural fluid eosinophilia caused by platelet-activating factor (PAF), the pleural cavity became hyporesponsive to allergen-induced protein exudation and to the parallel reduction in the number of intact mast cells. 4 These hyporesponsive animals presented a significant augmentation in the pleural effluent level of prostaglandin E(2) (PGE(2)), which increased with increasing numbers of eosinophils in the pleural cavity. Furthermore, pretreatment with either indomethacin or aspirin failed to modify allergen-induced exudation but reversed the exudatory hyporesponsiveness associated with eosinophil recruitment. 5 Allergic exudation was clearly down-regulated by the following pretreatments: (i) PGE(2) (10 mu g/cavity, i.pl.) plus rolipram (40 mu g/cavity, i.pl.), (ii) misoprostol (200 mu g kg(-1), p.o.) or (iii) dibutyryl cyclic AMP (80 mu g/cavity, i.pl.). 6 We conclude that prostaglandins may be implicated in the eosinophil-mediated inhibition of allergic pleurisy, probably acting via cyclic AMP signalling pathway activation.