Porous silicon-graphene oxide core-shell nanoparticles for targeted delivery of siRNA to the injured brain

被引：2

作者：

Joo, Jinmyoung ^{[1
,12
]}

Kwon, Ester J. ^{[2
]}

Kang, Jinyoung ^{[3
]}

Skalak, Matthew ^{[2
]}

Anglin, Emily J. ^{[1
]}

Mann, Aman P. ^{[4
]}

Ruoslahti, Erkki ^{[4
,5
,6
]}

Bhatia, Sangeeta N. ^{[2
,7
,8
,9
,10
,11
]}

Sailor, Michael J. ^{[1
,3
]}

机构：

[1] Univ Calif San Diego, Dept Chem, La Jolla, CA 92093 USA

[2] MIT, Harvard MIT Div Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[3] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA

[4] Sanford Burnham Prebys Med Discovery Inst, Canc Res Ctr, La Jolla, CA 92037 USA

[5] Univ Calif Santa Barbara, Ctr Nanomed, Santa Barbara, CA 93106 USA

[6] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

[7] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

[8] MIT, David H Koch Inst Integrat Canc Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[9] Brigham & Womens Hosp, Dept Med, 75 Francis St, Boston, MA 02115 USA

[10] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[11] Broad Inst Harvard & MIT, Cambridge, MA 02142 USA

[12] Univ Ulsan, Asan Med Ctr, Asan Inst Life Sci, Biomed Engn Res Ctr,Coll Med, Seoul 05505, South Korea

来源：

NANOSCALE HORIZONS | 2016年 / 1卷 / 05期

关键词：

SMALL INTERFERING RNA; IN-VIVO; GENE DELIVERY; CATIONIC LIPIDS; DRUG-DELIVERY; CANCER; CHITOSAN; GOLD; CODELIVERY; POLYMERS;

D O I：

10.1039/c6nh00082g

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable mesoporous silicon nanoparticles (pSiNPs), where the voids of the nanoparticles are loaded with siRNA and the nanoparticles are encapsulated with graphene oxide nanosheets (GO-pSiNPs). The graphene oxide encapsulant delays release of the oligonucleotide payloads in vitro by a factor of 3. When conjugated to a targeting peptide derived from the rabies virus glycoprotein (RVG), the nanoparticles show 2-fold greater cellular uptake and gene silencing. Intravenous administration of the nanoparticles into brain-injured mice results in substantial accumulation specifically at the site of injury.

引用

页码：407 / 414

页数：8

共 60 条

[1] Graphene: Safe or Toxic? The Two Faces of the Medal [J].

Bianco, Alberto .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (19) :4986-4997

[2]

Canham LT, 2014, WOODH PUBL SER BIOM, V68, P3, DOI 10.1533/9780857097156.1.3

[3] Quantum-Dot-Conjugated Graphene as a Probe for Simultaneous Cancer-Targeted Fluorescent Imaging, Tracking, and Monitoring Drug Delivery [J].

Chen, Mei-Ling ;

He, Ye-Ju ;

Chen, Xu-Wei ;

Wang, Jian-Hua .

BIOCONJUGATE CHEMISTRY, 2013, 24 (03) :387-397

[4] Chitosan/siRNA Nanoparticles Encapsulated in PLGA Nanofibers for siRNA Delivery [J].

Chen, Menglin ;

Gao, Shan ;

Dong, Mingdong ;

Song, Jie ;

Yang, Chuanxu ;

Howard, Kenneth Alan ;

Kjems, Jorgen ;

Besenbacher, Flemming .

ACS NANO, 2012, 6 (06) :4835-4844

[5] Layer-by-Layer Assembly of Graphene Oxide Nanosheets on Polyamide Membranes for Durable Reverse-Osmosis Applications [J].

Choi, Wansuk ;

Choi, Jungkyu ;

Bang, Joona ;

Lee, Jung-Hyun .

ACS APPLIED MATERIALS & INTERFACES, 2013, 5 (23) :12510-12519

[6] RNAi-based nanomedicines for targeted personalized therapy [J].

Daka, Ala ;

Peer, Dan .

ADVANCED DRUG DELIVERY REVIEWS, 2012, 64 (13) :1508-1521

[7] Current prospects for RNA interference-based therapies [J].

Davidson, Beverly L. ;

McCray, Paul B., Jr. .

NATURE REVIEWS GENETICS, 2011, 12 (05) :329-340

[8] Evidence of RNAi in humans from systemically administered siRNA via targeted nanoparticles [J].

Davis, Mark E. ;

Zuckerman, Jonathan E. ;

Choi, Chung Hang J. ;

Seligson, David ;

Tolcher, Anthony ;

Alabi, Christopher A. ;

Yen, Yun ;

Heidel, Jeremy D. ;

Ribas, Antoni .

NATURE, 2010, 464 (7291) :1067-U140

[9] Interfering with disease: a progress report on siRNA-based therapeutics [J].

de Fougerolles, Antonin ;

Vornlocher, Hans-Peter ;

Maraganore, John ;

Lieberman, Judy .

NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (06) :443-453

[10] Layer-by-Layer Nanoparticles for Systemic Codelivery of an Anticancer Drug and siRNA for Potential Triple-Negative Breast Cancer Treatment [J].

Deng, Zhou J. ;

Morton, Stephen W. ;

Ben-Akiva, Elana ;

Dreaden, Erik C. ;

Shopsowitz, Kevin E. ;

Hammond, Paula T. .

ACS NANO, 2013, 7 (11) :9571-9584

← 1 2 3 4 5 6 →