Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans

被引：21

作者：

Ou, Jianfa ^{[1
]}

Xu, Ningning ^{[1
]}

Ernst, Patrick ^{[1
]}

Ma, Chao ^{[2
]}

Bush, Meredith ^{[2
]}

Goh, KahYong ^{[1
]}

Zhao, Jingbo ^{[3
]}

Zhou, Lufang ^{[1
]}

Yang, Shang-Tian ^{[3
]}

Liu, Xiaoguang ^{[1
]}

机构：

[1] Univ Alabama Birmingham, Dept Biomed Engn, 1670 Univ Blvd, Birmingham, AL 35233 USA

[2] Univ Alabama, Dept Chem & Biol Engn, 245 7th Ave, Tuscaloosa, AL 35401 USA

[3] Ohio State Univ, Dept Chem & Biomol Engn, 151 West Woodruff Ave, Columbus, OH 43210 USA

来源：

PROCESS BIOCHEMISTRY | 2017年 / 62卷

关键词：

Clostridium cellulovorans; Butanol fermentation; Consolidated bioprocessing; Corn residues; FIBROUS-BED BIOREACTOR; BUTYRIC-ACID; LIGNOCELLULOSIC BIOMASS; CRYSTALLINE CELLULOSE; BIOFUEL PRODUCTION; ETHANOL-PRODUCTION; FERMENTATION; ACETOBUTYLICUM; TYROBUTYRICUM; PRETREATMENT;

D O I：

10.1016/j.procbio.2017.07.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cellulolytic Clostridium cellulovorans has been engineered to produce n-butanol from low-value lignocellulosic biomass by consolidated bioprocessing (CBP). The objective of this study was to establish a robust cellulosic biobutanol production process using a metabolically engineered C. cellulovorans. First, various methods for the pretreatment of four different corn-based residues, including corn cob, corn husk, corn fiber, and corn bran, were investigated. The results showed that better cell growth and a higher concentration of n-butanol were produced from corn cob that was pretreated with sodium hydroxide. Second, the effects of different carbon sources (glucose, cellulose and corn cob), basal media and culture pH values on butanol production were evaluated in the fermentations performed in 2-L bioreactors to identify the optimal CBP conditions. Finally, the engineered C. cellulovorans produced butanol with final concentration > 3 g/L, yield > 0.14 g/g, and selectivity > 3 g/g from pretreated corn cob at pH 6.5 in CBP. This study showed that the fermentation process engineering of C. cellulovorans enabled a high butanol production directly from agricultural residues.

引用

页码：144 / 150

页数：7