Enhancement of Vindoline Production in Suspension Culture of the Catharanthus roseus Cell Line C20hi by Light and Methyl Jasmonate Elicitation

被引：14

作者：

He, Lihong ^{[1
,2
]}

Yang, Li ^{[1
]}

Tan, Ronghui ^{[1
]}

Zhao, Shujuan ^{[1
]}

Hu, Zhibi ^{[1
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, SATCM Key Lab New Resources & Qual Evaluat Chines, Shanghai Key Lab Compound Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

[2] Soochow Univ, Coll Med, Dept Cell Biol, Suzhou 215123, Peoples R China

来源：

ANALYTICAL SCIENCES | 2011年 / 27卷 / 12期

基金：

中国国家自然科学基金;

关键词：

INDOLE ALKALOID BIOSYNTHESIS; SECONDARY METABOLITES; DEACETYLVINDOLINE-4-O-ACETYLTRANSFERASE; TABERSONINE; PATHWAY;

D O I：

10.2116/analsci.27.1243

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effects of light and methyl jasmonate (MJ) on the transcription of biosynthetic genes as well as the accumulation of vindoline and catharanthine in Catharanthus roseus C20hi cell suspensions were studied. t16h (the gene encoding tabersonine 16-hydroxylase) could be induced by light and MJ, whereas d4h (the gene encoding deacetoxyvindoline 4-hydroxylase) could only be induced by light. Quantification by UPLC-MS showed that light significantly increased vindoline production in C20hi cells by about 0.49 - 5.51-fold more than that in controls, with the highest yield being 75.3 ng/g of dry weight. The biosynthesis of vindoline was further enhanced by combining MJ with light. The accumulation of catharanthine was not improved by either light or MJ elicitation. These results suggested that light and MJ could promote vindoline, but not catharanthine accumulation in C20hi cells.

引用

页码：1243 / 1248

页数：6

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