Photodynamic effect of protoporphyrin diarginate (PPArg2) on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts

被引：22

作者：

Grinholc, Mariusz ^{[1
,4
]}

Kawiak, Anna ^{[2
]}

Kurlenda, Julianna ^{[1
,4
]}

Graczyk, Alfreda ^{[3
]}

Bielawski, Krzysztof P. ^{[1
,4
]}

机构：

[1] Univ Gdansk, Intercollegiate Fac Biotechnol, Div Mol Diagnost, Dept Biotechnol, PL-80822 Gdansk, Poland

[2] Univ Gdansk, Intercollegiate Fac Biotechnol, Dept Biotechnol, Div Plant Protect & Biotechnol, PL-80822 Gdansk, Poland

[3] Mil Univ Technol, Inst Optoelect, Warsaw, Poland

[4] Med Univ Gdansk, PL-80822 Gdansk, Poland

来源：

ACTA BIOCHIMICA POLONICA | 2008年 / 55卷 / 01期

关键词：

antimicrobial photodynamic inactivation; cytotoxicity; fibroblasts; MRSA; phototoxicity;

D O I：

10.18388/abp.2008_3204

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The worldwide rise in the antibiotic resistance of bacteria forces the development of alternative antimicrobial treatments. A potential approach is photodynamic inactivation (PDI). The aim of the present study was to determine the phototoxicity of protoporphyrin diarginate (PPArg(2)) against methicillin-resistant Staphylococcus aureus and human dermal fibroblasts. Different concentrations (0 to 20 mu M) of PPArg(2) and light dose of 6 J cm(-2) were tested. Cell viability was evaluated using the methylthiazoletetrazolium (MTT) assay. Incubation with 10 mu M followed by illumination yielded a 3.6 log(10)-unit reduction in the viable count for Staphylococcus aureus. At the same experimental conditions, only 22.5% of the fibroblasts were photoinactivated. Protoporphyrin diarginate at concentrations up to 20 mu M demonstrated no toxicity towards S. aureus or fibroblasts when not irradiated. These results suggest that the protoporphyrin diarginate exerts a high bactericidal effect against methicillin-resistant S. aureus strain without harming eukaryotic cells.

引用

页码：85 / 90

页数：6

共 33 条

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