A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans

被引：42
作者：
Chen, Haiqiang [1 ,2 ]
Wang, Shuang [1 ,2 ]
Zhou, Aimei [1 ,2 ]
Miao, Jianyin [1 ,2 ]
Liu, Jingyi [1 ,2 ]
Benjakul, Soottawat [3 ]
机构：
[1] South China Agr Univ, Coll Food Sci, Guangzhou 510642, Peoples R China
[2] Guangdong Prov Key Lab Nutraceut & Funct Foods, Guangzhou 510642, Peoples R China
[3] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Songkhla, Thailand
来源：
JOURNAL OF FUNCTIONAL FOODS | 2020年 / 68卷
关键词：
Antioxidative peptide; Purification; Caenorhabditis elegans; Anti-aging; Round scad; OXIDATIVE STRESS; ANTIAGING ACTIVITIES; BIOACTIVE PEPTIDES; IN-VITRO; PURIFICATION; IDENTIFICATION; HEALTHSPAN; RESISTANCE; ALCALASE; ABTS;
D O I：
10.1016/j.jff.2020.103907
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The residue rich in protein (up to 88.78%) obtained from round scad (Decapterus maruadsi) after oil extraction, was hydrolyzed by Alcalase to produce antioxidative peptides. The hydrolysate was separated by ultrafiltration, Sephadex G-15 and RP-HPLC, and the active fractions were screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6 sulphonic acid) diammonium salt (ABTS), reducing power and oxygen radical absorbance capacity (ORAC). The obtained peptide with the highest antioxidative activity was then analyzed by HPLC and LC-MS/MS and its anti-aging effect was examined using Caenorhabditis elegans (C. elegans) model. The peptide (Ile-Leu-Gly-Ala-Thr-Ile-Asp-Asn-Ser-Lys, 1153.4 Da), identified by LC-MS/MS showed the highest antioxidant activity. C. elegans fed with the selected peptide had longer lifespan, higher survival rate, superoxide dismutase (SOD) and catalase (CAT) activities than the control group (P<0.05), demonstrating the promising anti-aging effects of the antioxidant peptide from defatted round scad.
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页数：11
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