Biolabeling Hematopoietic System Cells Using Near-Infrared Fluorescent Gold Nanoclusters

Highly bright fluorescent gold nanoclusters (Au NCs) have been prepared by one-step reduction of aqueous precursor solution in the presence of multidentate thioether-terminated poly(methacrylic acid) (PTMP-PMAA). The fluorescence quantum yield of the resultant Au NCs is 4.8% higher than that of the similarly sized Au NCs prepared by the etching method (1.8-4.0%). These Au NCs show excellent photostability and have been successfully applied to label the hematopoietic cells first. The results show that Au NCs were endocytosed by the cancer cells significantly more than the normal cells, in comparison with control experiments labeled with fluorescent quantum dots (CdTe). The cytotoxicity experiments demonstrate the excellent biocompatibility of Au NCs, proven by a relatively lower cytotoxicity than CdTe. These robust near-infrared Au NCs show great potential in biolabeling, tracking, and imaging of other cells and diseases, especially in the diagnosis and treatment of chronic myeloid leukemia.