Towards high-throughput flim for protein-protein interaction screening of live cells and tissue microarrays

被引：3

作者：

Barber, P. R. ^{[1
]}

Pierce, G. P. ^{[1
]}

Ameer-Beg, S. M. ^{[2
]}

Matthews, D. R. ^{[2
]}

Carlin, L. M. ^{[2
]}

Keppler, M. ^{[2
]}

Kelleher, M. ^{[2
]}

Festy, F. ^{[2
,3
]}

Gillett, C. ^{[4
]}

Springall, R.

Ng, T. C.

Vojnovic, B. ^{[1
]}

机构：

[1] Univ Oxford, Mt Vernon Hosp, Gray Canc Inst, Northwood HA6 2JR, Middx, England

[2] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England

[3] Kings Coll London, Biomimet & Biophoton Res Grp, London WC2R 2LS, England

[4] Kings Coll London, Breast Tissue & Data Bank, London WC2R 2LS, England

来源：

2008 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1-4 | 2008年

基金：

英国工程与自然科学研究理事会;

关键词：

FRET/FLIM; high-content screening; tissue micro arrays; protein-protein interactions;

D O I：

10.1109/ISBI.2008.4541006

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.

引用

页码：356 / +

页数：2

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