Analysing ambiguities in trypanosomatids taxonomy by barcoding

被引：3

作者：

Boucinha, Carolina ^{[1
]}

Caetano, Amanda R. ^{[1
]}

Santos, Helena L. C. ^{[1
]}

Healers, Raphael ^{[2
]}

Vikkula, Miikka ^{[2
]}

Branquinha, Marta Helena ^{[3
]}

Souza dos Santos, Andre Luis ^{[3
]}

Grellier, Philippe ^{[4
]}

Morelli, Karina Alessandra ^{[1
,5
]}

d'Avila-Levy, Claudia M. ^{[1
,2
]}

机构：

[1] Fundacao Oswaldo Cruz Fiocruz, Inst Oswaldo Cruz, Lab Estudos Integrados Protozool, Colecao Protozoarios Fiocruz, Rio De Janeiro, RJ, Brazil

[2] Univ Louvain, Duve Inst, Lab Human Mol Genet, Brussels, Belgium

[3] Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes, Rio De Janeiro, Brazil

[4] Museum Natl Hist Nat, Unite Mol Commun & Adaptat Microorganisme, Paris, France

[5] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Comes, Dept Ecol, Rio De Janeiro, RJ, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2020年 / 115卷

关键词：

barcode; barcoding; Kinetoplastea; taxonomy; Trypanosomatida; Trypanosornatidae; voucher; SP-N PROTOZOA; AXENIC CULTIVATION; INSECT TRYPANOSOMATIDS; PHYTOMONAS SP; PHYLOGENETIC-RELATIONSHIPS; FLAGELLATE PARASITES; SALIVARY-GLANDS; KINETOPLAST DNA; HERPETOMONAS; CRITHIDIA;

D O I：

10.1590/0074-02760200504

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

BACKGROUND Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity. METHODS We sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br. OBJECTIVE The main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidac genera, to generate phylogenetic trees that can simplify new isolates screenings. FINDINGS A DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits. MAIN CONCLUSIONS This survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections.

引用

页码：1 / 14

页数：14

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[21] HERPETOMONAS-MEGASELIAE SP-N (PROTOZOA-TRYPANOSOMATIDAE) FROM MEGASELIA-SCALARIS (LOEW, 1866) SCHMITZ, 1929 (DIPTERA-PHORIDAE) [J].