miR-181b promotes the oncogenesis of renal cell carcinoma by targeting TIMP3

被引:0
作者
Zou, Yuhua [1 ]
Zhang, Lei [2 ]
Zhong, Xin [3 ]
机构
[1] Gannan Med Univ, Dept Urol, Affiliated Hosp 1, Ganzhou 341000, Peoples R China
[2] First Peoples Hosp Xiangtan City, Dept Urol, Xiangtan 411101, Peoples R China
[3] Shenzhen SAMII Med Ctr, Dept Gen Surg, Shenzhen 518118, Peoples R China
关键词
microRNA; microRNA-181b; Tissue inhibitors of metalloproteinase 3; Renal cell carcinoma; 3 '-UTR region; TISSUE INHIBITOR; CANCER; PROLIFERATION; EXPRESSION; METALLOPROTEINASE-3; METASTASIS; RESISTANCE; SUNITINIB; INVASION;
D O I
10.32604/biocell.2022.018167
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Renal cell carcinoma (RCC) has a poor prognosis due to limited diagnosis and treatment. Thus, it is necessary to find novel prognostic biomarkers and therapeutic targets. The aberrant expression of microRNAs plays an important role in RCC oncogenesis. Tissue inhibitors of metalloproteinase 3 (TIMP3) acts as a downstream target of miR-181b. The aim of this study was to understand the role and molecular mechanism of miR-181b in RCC oncogenesis. The results showed that miR-181b expression was significantly higher in RCC tumour tissues, especially in those with significant invasion or metastasis. miR-181b overexpression promoted proliferation and migration of the RCC cell line 786-O, while miR-181b knockdown had the opposite effect. In addition, miR-181b was inversely correlated with TIMP3 expression in RCC tumour tissues. miR-181b overexpression reduced TIMP3 expression in RCC cell line 786-O or OS-RC-2, while miR-18 lb knockdown had the inverse effect. Mechanistically, a luciferase reporter assay confirmed the binding sites of miR-181b on the 3'-UTR of TIMP3, confirming the targeting effect of miR-181b on TIMP3. Overall, miR-181b promotes the development and progression of RCC by targeting TIMP3 expression, indicating the potential use of miR-181b in the diagnosis and treatment of RCC.
引用
收藏
页码:1309 / 1317
页数:9
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