Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil

被引:12
作者
Prado Martins, Rodrigo [1 ]
da Silva, Maria Cristina [1 ]
Dutra, Valeria [1 ]
Nakazato, Luciano [1 ]
Leite, Domingos da Silva [2 ]
机构
[1] Univ Fed Mato Grosso, Dept Clin Med Vet, Campo Grande, Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Microbiol & Imunol, BR-13081970 Campinas, SP, Brazil
来源
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES | 2011年 / 5卷 / 02期
关键词
STEC; ETEC; PCR; slaughterhouse; Mato Grosso; VIRULENCE FACTORS; HEALTHY CATTLE; PARANA STATE; DIARRHEA; CONTAMINATION; O157-H7; INFECTION; ANIMALS; PIGLETS; SHEEP;
D O I
10.3855/jidc.1217
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Introduction: This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil. Methodology: Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays. Results: It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1. Conclusion: The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.
引用
收藏
页码:123 / 126
页数:4
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