Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester

被引：133
作者：
Gunawan, ER
Basri, M [1 ]
Rahman, MBA
Salleh, AB
Rahman, RNZA
机构：
[1] Univ Putra Malaysia, Fac Sci, Serdang 43400, Selangor, Malaysia
[2] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Serdang 43400, Selangor, Malaysia
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2005年 / 37卷 / 07期
关键词：
response surface methodology (RSM); central composite rotatable design (CCRD); palm oil; lipozyme; alcoholysis; wax ester;
D O I：
10.1016/j.enzmictec.2005.04.010
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (2.5-10 h), temperature (30-70 degrees C), amount of enzyme (0.1-0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1: 1-1:5) on the percentage yield of wax esters. The optimum conditions derived via RSM were: reaction time 7.38h, temperature 53.9 degrees C, amount of enzyme 0.149g, and substrate molar ratio 1:3.41. The actual experimental yield was 84.6% under optimum condition, which compared well to the maximum predicted value of 85.4%. (c) 2005 Elsevier Inc. All rights reserved.
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收藏
页码：739 / 744
页数：6
相关论文
共 22 条
[1]   FORMATION OF A JOJOBA OIL ANALOG BY ESTERIFICATION OF OLEIC-ACID USING ZEOLITES AS CATALYST [J].
ARACIL, J ;
MARTINEZ, M ;
SANCHEZ, N ;
CORMA, A .
ZEOLITES, 1992, 12 (03) :233-236
[2]   Optimisation of kojic acid monolaurate synthesis with lipase PS from Pseudomonas cepacia [J].
Chen, CS ;
Liu, KJ ;
Lou, YH ;
Shieh, CJ .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2002, 82 (06) :601-605
[3]   Studies on the optimized lipase-catalyzed biosynthesis of cis-3-hexen-l-yl acetate in n-hexane [J].
Chiang, WD ;
Chang, SW ;
Shieh, CJ .
PROCESS BIOCHEMISTRY, 2003, 38 (08) :1193-1199
[4]  
COHRAN WG, 2002, EXPT DESIGN
[5]   Enzymatic alcoholysis of triolein to produce wax ester [J].
Hadzir, NM ;
Basri, M ;
Rahman, MBA ;
Razak, CNA ;
Rahman, RNZA ;
Salleh, AB .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2001, 76 (05) :511-515
[6]   Enzymatic synthesis of wax esters from rapeseed fatty acid methyl esters and a fatty alcohol [J].
Hallberg, ML ;
Wang, DB ;
Härröd, M .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1999, 76 (02) :183-187
[7]   Response surface methodological approach for the synthesis of isobutyl isobutyrate [J].
Hamsaveni, DR ;
Prapulla, SG ;
Divakar, S .
PROCESS BIOCHEMISTRY, 2001, 36 (11) :1103-1109
[8]   Thermostability of porcine pancreas lipase in non-aqueous media [J].
Kiran, KR ;
Babu, CVS ;
Divakar, S .
PROCESS BIOCHEMISTRY, 2001, 36 (8-9) :885-892
[9]   A central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level [J].
Kiran, KR ;
Manohar, B ;
Divakar, S .
ENZYME AND MICROBIAL TECHNOLOGY, 2001, 29 (2-3) :122-128
[10]  
Krishna SH, 2001, PROCESS BIOCHEM, V37, P9
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