Activation of p21-activated protein kinase alpha (alpha PAK) by hyperosmotic shock in neonatal ventricular myocytes

被引：40

作者：

Clerk, A ^{[1
]}

Sugden, PH ^{[1
]}

机构：

[1] NATL HEART & LUNG INST,IMPERIAL COLL SCH MED,DIV CARDIAC MED,LONDON SW3 6LY,ENGLAND

来源：

FEBS LETTERS | 1997年 / 403卷 / 01期

关键词：

p21-activated protein kinase alpha; hyperosmotic shock; cardiac myocyte;

D O I：

10.1016/S0014-5793(97)00020-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The p21-activated protein kinases (PAKs) may participate in signalling from Cdc42/Rac1 to the stress-regulated MAPKs (SAPKs/JNKs and p38-/HOG-1-related-MAPKs). We characterized the expression and regulation of alpha PAK in cultured ventricular myocytes. alpha PAK was specifically immunoprecipitated from myocyte extracts. High basal alpha PAK activity was detected in unstimulated myocytes. Its activity was increased rapidly (< 30 s) by hyperosmotic shock in the presence of okadaic acid, and was maximal by 3 min (187 +/- 7% relative to unstimulated cells). Endothelin-1 and interleukin-1 beta, which also activate SAPKs/JNKs, did not increase alpha PAK activity and presumably act through different PAK isoforms or other mechanisms. (C) 1997 Federation of European Biochemical Societies.

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页码：23 / 25

页数：3

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