Optimization of L-lactic acid production of rhizopus oryzae mutant RLC41-6 by ion beam implantation at low-energy

被引:0
|
作者
Zhou, XH [1 ]
Ge, CM [1 ]
Yao, JM [1 ]
Pan, RR [1 ]
Yu, ZL [1 ]
机构
[1] Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
关键词
ion implantation; fermentation; L(+)-lactic acid; Rhizopus oryzae;
D O I
暂无
中图分类号
O35 [流体力学]; O53 [等离子体物理学];
学科分类号
070204 ; 080103 ; 080704 ;
摘要
In order to obtain an industrial strain with a higher L(+)-lactic acid yield, the strain Rhizopus oryzae RF3608 was mutated by means of nitrogen ion beam implantation and the mutant strain RLC41-6 was isolated. Under optimal conditions the yield of L(+)-lactic acid produced in a shake-flask reached 133 g/L similar to 137 g/L after 36 h cultivation, indicating that the conversion rate based on glucose was as high as 88% similar to 91% and the productivity was 3.75 g/L center dot h. It was almost a 115% increase in lactic acid production compared with the original strain RF3608.
引用
收藏
页码:3078 / 3080
页数:3
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