Chronic inflammation and CD16+ natural killer cell zeta-chain downregulation in hemodialysis patients

被引:26
作者
Eleftheriadis, Theodoros [1 ]
Kartsios, Charalambos [2 ]
Yiannaki, Efi [2 ]
Kazila, Polizo [3 ]
Antoniadi, Georgia [1 ]
Liakopoulos, Vassilios [1 ]
Markala, Dimitra [2 ]
机构
[1] Gen Hosp Serres, Dept Nephrol, GR-62100 Serres, Greece
[2] Theagen Anticanc Hosp, Hematol Lab, Thessaloniki, Greece
[3] Theagen Anticanc Hosp, Biochem Lab, Thessaloniki, Greece
关键词
hemodialysis; immune response; inflammation; natural killer cell; zeta chain;
D O I
10.1159/000130068
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Natural killer (NK) cell activity is decreased in hemodialysis (HD) patients. Zeta-chain phosphorylation is an early event that follows the triggering of some NK cellactivating receptors. NK cell zeta-chain is downregulated in patients with cancer due to chronic inflammation. HD is also a chronic inflammatory state. NK cell zeta- chain expression in HD was evaluated. Patients and Methods: Thirty-three HD patients and 30 healthy volunteers were enrolled into the study. The CD3-CD16+ subpopulation was examined, since it corresponds to 90% of all NK cells and has the highest cytotoxicity. NK cell count and zeta- chain mean fluorescence intensity were evaluated with flow cytometry. The inflammatory markers C- reactive protein, IL-6 and tumor necrosis factor-alpha were measured with ELISA. Results: The inflammatory markers were increased in HD patients. NK cell count did not differ between HD patients and healthy volunteers. NK cell zeta- chain mean fluorescence intensity was decreased in the patient group. Conclusions: Chronic inflammation could be responsible for the NK cell zetachain downregulation in HD patients, contributing to the decreased NK cell activity. Copyright (c) 2008 S. Karger AG, Basel.
引用
收藏
页码:317 / 321
页数:5
相关论文
共 28 条
[1]   DEPRESSED NATURAL-KILLER CELL-ACTIVITY IN UREMIA - EVIDENCE FOR IMMUNOSUPPRESSIVE FACTOR IN UREMIC SERA [J].
ASAKA, M ;
IIDA, H ;
IZUMINO, K ;
SASAYAMA, S .
NEPHRON, 1988, 49 (04) :291-295
[2]   Tcr ζ-chain downregulation:: Curtailing an excessive inflammatory immune response [J].
Baniyash, M .
NATURE REVIEWS IMMUNOLOGY, 2004, 4 (09) :675-687
[3]   Inflammation-associated immune suppression in cancer: The roles played by cytokines, chemokines and additional mediators [J].
Ben-Baruch, A .
SEMINARS IN CANCER BIOLOGY, 2006, 16 (01) :38-52
[4]   NEGATIVE EFFECT OF UREMIA AND CUPROPHANE HEMODIALYSIS ON NATURAL-KILLER-CELLS [J].
CALA, S ;
MAZURAN, R ;
KORDIC, D .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 1990, 5 (06) :437-440
[5]   The biology of human natural killer-cell subsets [J].
Cooper, MA ;
Fehniger, TA ;
Caligiuri, MA .
TRENDS IN IMMUNOLOGY, 2001, 22 (11) :633-640
[6]   Inflammation and cancer [J].
Coussens, LM ;
Werb, Z .
NATURE, 2002, 420 (6917) :860-867
[7]   Antigen phenotype and cytotoxic activity of natural killer cells in hemodialysis patients [J].
Gascon, A ;
Orfao, A ;
Lerma, JL ;
Ciudad, J ;
Lopez, A ;
Hernandez, MD ;
Tabernero, JM .
AMERICAN JOURNAL OF KIDNEY DISEASES, 1996, 27 (03) :373-379
[8]   Influence of dialysis with polyamide vs haemophan haemodialysers on monokines and complement activation during a 4-month long-term study [J].
Girndt, M ;
Heisel, O ;
Köhler, H .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 1999, 14 (03) :676-682
[9]   PRODUCTION OF INTERLEUKIN-6, TUMOR-NECROSIS-FACTOR-ALPHA AND INTERLEUKIN-10 IN-VITRO CORRELATES WITH THE CLINICAL IMMUNE DEFECT IN CHRONIC-HEMODIALYSIS PATIENTS [J].
GIRNDT, M ;
KOHLER, H ;
SCHIEDHELMWEICK, E ;
SCHLAAK, JF ;
ZUMBUSCHENFELDE, KHM ;
FLEISCHER, B .
KIDNEY INTERNATIONAL, 1995, 47 (02) :559-565
[10]   DIFFERENTIAL EFFECT OF HEMODIALYSIS MEMBRANES ON HUMAN-LYMPHOCYTE NATURAL-KILLER FUNCTION [J].
KAY, NE ;
RAIJ, L .
ARTIFICIAL ORGANS, 1987, 11 (02) :165-167