Destabilization of DNA and RNA G-quadruplex structures formed by GGA repeat due to N6-methyladenine modification

被引：7
作者：
Iwasaki, Yuka [1 ]
Ookuro, Yurino [2 ]
Iida, Keisuke [3 ]
Nagasawa, Kazuo [4 ]
Yoshida, Wataru [1 ,2 ]
机构：
[1] Tokyo Univ Technol, Grad Sch Bion, 1404-1 Katakura, Hachioji, Tokyo 1920982, Japan
[2] Tokyo Univ Technol, Sch Biosci & Biotechnol, 1404-1 Katakura, Hachioji, Tokyo 1920982, Japan
[3] Chiba Univ, Dept Chem, 1-33 Yayoi, Inage, Chiba 2638522, Japan
[4] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, 2-24-16 Naka Cho, Koganei, Tokyo 1848588, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2022年 / 597卷
关键词：
G-quadruplex; GGA repeat; N-6-methyladenine; Thermal stability; NUCLEIC-ACID CONFORMATIONS; METHYLATION; PROMOTER; IDENTIFICATION; N-6-ADENINE; STABILITY; SEQUENCE; APTAMER; PROTEIN; HEPTAD;
D O I：
10.1016/j.bbrc.2022.01.123
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
N-6-methyladenine (m(6)A) is the most abundant RNA modification in eukaryotic RNA. Further, m(6)A has been identified in the genomic DNA of both eukaryotes and prokaryotes. The G-quadruplex (G4) structure is a non-canonical nucleic acid structure formed by the stacking of G:G:G:G tetrads. In this study, we evaluated the effect of m(6)A modifications on G4 structures formed by GGA repeat oligonucleotides, d(GGA)8, d(GGA)(4), and r(GGA)(4). The d(GGA)(8) forms an intramolecular tetrad:heptad:heptad:tetrad G4 structure, while d(GGA)(4) forms a dimerized intermolecular tetrad:heptad:heptad:tetrad G4 structure. r(GGA)(4) forms a dimerized intermolecular tetrad:hexad:hexad:tetrad G4 structure. Circular dichroism melting analysis demonstrated that (1) m(6)A modifications destabilized the G4 structure formed by d(GGA)(8), (2) m(6)A modification at A3 disrupted the G4 structure formed by d(GGA)(4), and (3) m(6)A modification at A3 destabilized the G4 structure formed by r(GGA)4. m(6)A modifications may be involved in controlling G(4) structure formation to regulate biological functions. (c) 2022 Elsevier Inc. All rights reserved.
引用
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页码：134 / 139
页数：6
相关论文
共 43 条
  • [1] HNRNPA2B1 Is a Mediator of m6A-Dependent Nuclear RNA Processing Events
    Alarcon, Claudio R.
    Goodarzi, Hani
    Lee, Hyeseung
    Liu, Xuhang
    Tavazoie, Saeed
    Tavazoie, Sohail F.
    [J]. CELL, 2015, 162 (06) : 1299 - 1308
  • [2] Quadruplex DNA: sequence, topology and structure
    Burge, Sarah
    Parkinson, Gary N.
    Hazel, Pascale
    Todd, Alan K.
    Neidle, Stephen
    [J]. NUCLEIC ACIDS RESEARCH, 2006, 34 (19) : 5402 - 5415
  • [3] High-throughput sequencing of DNA G-quadruplex structures in the human genome
    Chambers, Vicki S.
    Marsico, Giovanni
    Boutell, Jonathan M.
    Di Antonio, Marco
    Smith, Geoffrey P.
    Balasubramanian, Shankar
    [J]. NATURE BIOTECHNOLOGY, 2015, 33 (08) : 877 - +
  • [4] Solution Structure of an Intramolecular (3+1) Human Telomeric G-Quadruplex Bound to a Telomestatin Derivative
    Chung, Wan Jun
    Heddi, Brahim
    Tera, Masayuki
    Iida, Keisuke
    Nagasawa, Kazuo
    Anh Tuan Phan
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (36) : 13495 - 13501
  • [5] Exploration of the Structure and Recognition of a G-quadruplex in the her2 Proto-oncogene Promoter and Its Transcriptional Regulation
    Cui, Xiaojie
    Chen, Han
    Zhang, Qiang
    Xu, Ming
    Yuan, Gu
    Zhou, Jiang
    [J]. SCIENTIFIC REPORTS, 2019, 9 (1)
  • [6] EFFECTS OF METHYLATION ON STABILITY OF NUCLEIC-ACID CONFORMATIONS - STUDIES AT MONOMER LEVEL
    ENGEL, JD
    HIPPEL, PHV
    [J]. BIOCHEMISTRY, 1974, 13 (20) : 4143 - 4158
  • [7] ENGEL JD, 1978, J BIOL CHEM, V253, P927
  • [8] N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas
    Fu, Ye
    Luo, Guan-Zheng
    Chen, Kai
    Deng, Xin
    Yu, Miao
    Han, Dali
    Hao, Ziyang
    Liu, Jianzhao
    Lu, Xingyu
    Dore, Louis C.
    Weng, Xiaocheng
    Ji, Quanjiang
    Mets, Laurens
    He, Chuan
    [J]. CELL, 2015, 161 (04) : 879 - 892
  • [9] DNA Methylation on N6-Adenine in C-elegans
    Greer, Eric Lieberman
    Blanco, Mario Andres
    Gu, Lei
    Sendinc, Erdem
    Liu, Jianzhao
    Aristizabal-Corrales, David
    Hsu, Chih-Hung
    Aravind, L.
    He, Chuan
    Shi, Yang
    [J]. CELL, 2015, 161 (04) : 868 - 878
  • [10] Enhancer-promoter interaction facilitated by transiently forming G-quadruplexes
    Hegyi, Hedi
    [J]. SCIENTIFIC REPORTS, 2015, 5 : 1 - 6
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