Phenotypic characterization of skeletal abnormalities of Osteopotentia mutant mice by micro-CT: a descriptive approach with emphasis on reconstruction techniques

被引:4
作者
Roemer, Frank W. [1 ,6 ,7 ,8 ]
Mohr, Andreas [8 ,9 ]
Guermazi, Ali [7 ,8 ]
Jiang, Yebin [8 ,10 ]
Schlechtweg, Philipp [2 ]
Genant, Harry K. [3 ,8 ]
Sohaskey, Michael L. [4 ,5 ]
机构
[1] Boston Univ, Med Ctr, Dept Radiol, Boston, MA 02118 USA
[2] Univ Erlangen Nurnberg, Dept Radiol, Erlangen, Germany
[3] CCBR SYNARC Inc, San Francisco, CA 94105 USA
[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[5] Univ Calif Berkeley, Ctr Integrat Genom, Berkeley, CA 94720 USA
[6] Klinikum Augsburg, Dept Radiol, D-86156 Augsburg, Germany
[7] Boston Univ, Quantitat Imaging Ctr, Sch Med, Boston, MA 02118 USA
[8] Univ Calif San Francisco, Osteoporosis & Arthrit Res Grp, San Francisco, CA 94117 USA
[9] Sligo Gen Hosp, Dept Radiol, Sligo, Ireland
[10] Univ Michigan, Sch Med, Dept Radiol, Musculoskeletal Div,Osteoporosis & Arthrit Lab, Ann Arbor, MI 48109 USA
关键词
Animal model; Micro CT; Bone; Radiography; Fracture; Osteogenesis imperfecta; BONE; FEASIBILITY; FRACTURES; FEMUR; SPINE;
D O I
10.1007/s00256-010-1082-2
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Purpose The novel protein osteopotentia (Opt) has recently been described as an essential regulator of postnatal osteoblast maturation and might possibly be responsible for some of the rarer types of osteogenesis imperfecta. Our aim was the evaluation of micro CT for the qualitative morphological assessment of skeletal abnormalities of Osteopotentia-mutant mice in comparison to radiography and histology. Materials and methods Four homozygous mice with insertional mutations in the Opt gene and three wild-type controls were examined ex vivo using radiography and micro-CT. Two of the homozygous animals were evaluated histologically (trichrome reagent). For the micro-CT evaluation three-dimensional (3D) surface reconstructions and two-dimensional (2D) multiplanar reformations (MPRs) were applied. Results The Opt-homozygous mice exhibited severe growth. The radiographic examinations showed osteopenia and fractures with hypertrophic callus formation and pseudarthroses of the forelimbs and ribs. Micro-CT confirmed these findings and was able to demonstrate additional fractures especially at smaller bones such as the metacarpals and phalanges. Additional characterization and superior delineation of cortices and fracture fragments was achieved by 2D MPRs. Histological correlation verified several of these imaging findings. Conclusion Micro-CT is able to screen Opt-mutant mice for osseous pathologies and furthermore characterize these anomalies. The modality seems superior to conventional radiography, but is not able to demonstrate cellular pathology. However, histology is destructive and more time- and material-consuming than micro-CT. Additional information may be gathered by 2D MPRs.
引用
收藏
页码:1073 / 1078
页数:6
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