Single-frame 3D fluorescence microscopy with ultraminiature lensless FlatScope

被引:163
作者
Adams, Jesse K. [1 ,2 ,3 ]
Boominathan, Vivek [2 ,3 ]
Avants, Benjamin W. [2 ]
Vercosa, Daniel G. [1 ,2 ]
Ye, Fan [2 ,3 ]
Baraniuk, Richard G. [2 ,3 ]
Robinson, Jacob T. [1 ,2 ,3 ,4 ,5 ]
Veeraraghavan, Ashok [1 ,2 ,3 ]
机构
[1] Rice Univ, Appl Phys Program, 6100 Main St, Houston, TX 77005 USA
[2] Rice Univ, Dept Elect & Comp Engn, Houston, TX 77005 USA
[3] Rice Univ, Nanophoton Computat Imaging & Sensing Lab, Houston, TX 77005 USA
[4] Rice Univ, Dept Bioengn, Houston, TX 77005 USA
[5] Baylor Coll Med, Dept Neurosci, One Baylor Plaza, Houston, TX 77030 USA
来源
SCIENCE ADVANCES | 2017年 / 3卷 / 12期
基金
美国国家科学基金会;
关键词
CHIP; FILTER;
D O I
10.1126/sciadv.1701548
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Modern biology increasingly relies on fluorescence microscopy, which is driving demand for smaller, lighter, and cheaper microscopes. However, traditional microscope architectures suffer from a fundamental trade-off: As lenses becomesmaller, theymust either collect less light or image a smaller field of view. To break this fundamental trade-off between device size and performance, we present a new concept for three-dimensional (3D) fluorescence imaging that replaces lenses with an optimized amplitude mask placed a few hundred micrometers above the sensor and an efficient algorithm that can convert a single frame of captured sensor data into high-resolution 3D images. The result is FlatScope: perhaps the world's tiniest and lightest microscope. FlatScope is a lensless microscope that is scarcely larger than an image sensor (roughly 0.2 g in weight and less than 1mmthick) and yet able to produce micrometer-resolution, high-frame rate, 3D fluorescence movies covering a total volume of several cubic millimeters. The ability of FlatScope to reconstruct full 3Dimages froma single frame of captured sensor data allows us to image 3Dvolumes roughly 40,000 times faster than a laser scanning confocal microscope while providing comparable resolution. We envision that this new flat fluorescence microscopy paradigm will lead to implantable endoscopes that minimize tissue damage, arrays of imagers that cover large areas, and bendable, flexible microscopes that conform to complex topographies.
引用
收藏
页数:9
相关论文
共 38 条
  • [1] FlatCam: Thin, Lensless Cameras Using Coded Aperture and Computation
    Asif, M. Salman
    Ayremlou, Ali
    Sankaranarayanan, Aswin
    Veeraraghavan, Ashok
    Baraniuk, Richard G.
    [J]. IEEE TRANSACTIONS ON COMPUTATIONAL IMAGING, 2017, 3 (03): : 384 - 397
  • [2] A Fast Iterative Shrinkage-Thresholding Algorithm for Linear Inverse Problems
    Beck, Amir
    Teboulle, Marc
    [J]. SIAM JOURNAL ON IMAGING SCIENCES, 2009, 2 (01): : 183 - 202
  • [3] Imaging intracellular fluorescent proteins at nanometer resolution
    Betzig, Eric
    Patterson, George H.
    Sougrat, Rachid
    Lindwasser, O. Wolf
    Olenych, Scott
    Bonifacino, Juan S.
    Davidson, Michael W.
    Lippincott-Schwartz, Jennifer
    Hess, Harald F.
    [J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
  • [4] Lensless Imaging A computational renaissance
    Boominathan, Vivek
    Adams, Jesse K.
    Asif, M. Salman
    Avants, Benjamin W.
    Robinson, Jacob T.
    Baraniuk, Richard G.
    Sankaranarayanan, Aswin C.
    Veeraraghavan, Ashok
    [J]. IEEE SIGNAL PROCESSING MAGAZINE, 2016, 33 (05) : 23 - 35
  • [5] Brady D. J., 2009, Optical imaging and spectroscopy
  • [6] Robust Principal Component Analysis?
    Candes, Emmanuel J.
    Li, Xiaodong
    Ma, Yi
    Wright, John
    [J]. JOURNAL OF THE ACM, 2011, 58 (03)
  • [7] Lensfree Fluorescent On-Chip Imaging of Transgenic Caenorhabditis elegans Over an Ultra-Wide Field-of-View
    Coskun, Ahmet F.
    Sencan, Ikbal
    Su, Ting-Wei
    Ozcan, Aydogan
    [J]. PLOS ONE, 2011, 6 (01):
  • [8] Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects
    Coskun, Ahmet F.
    Sencan, Ikbal
    Su, Ting-Wei
    Ozcan, Aydogan
    [J]. OPTICS EXPRESS, 2010, 18 (10): : 10510 - 10523
  • [9] Wide field-of-view lens-free fluorescent imaging on a chip
    Coskun, Ahmet F.
    Su, Ting-Wei
    Ozcan, Aydogan
    [J]. LAB ON A CHIP, 2010, 10 (07) : 824 - 827
  • [10] Lensless coded-aperture imaging with separable Doubly-Toeplitz masks
    DeWeert, Michael J.
    Farm, Brian P.
    [J]. OPTICAL ENGINEERING, 2015, 54 (02)