Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in 'Red Zaosu' pear fruits by interacting with MYB114

被引：147

作者：

Ni, Junbei ^{[1
,2
,3
]}

Bai, Songling ^{[1
,2
,3
]}

Zhao, Yuan ^{[1
,2
,3
]}

Qian, Minjie ^{[4
]}

Tao, Ruiyan ^{[1
,2
,3
]}

Yin, Lei ^{[1
,2
,3
]}

Gao, Ling ^{[5
]}

Teng, Yuanwen ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Dept Hort, Hangzhou 310058, Zhejiang, Peoples R China

[2] Minist Agr China, Key Lab Hort Plant Growth Dev & Qual Improvement, Hangzhou 310058, Zhejiang, Peoples R China

[3] Zhejiang Prov Key Lab Integrat Biol Hort Plants, Hangzhou 310058, Zhejiang, Peoples R China

[4] Orebro Univ, Sch Sci & Technol, Orebro, Sweden

[5] ACON Biotech Hangzhou Co Ltd, Hangzhou 310058, Zhejiang, Peoples R China

来源：

PLANT MOLECULAR BIOLOGY | 2019年 / 99卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Anthocyanin; Pear (Pyrus); ERF; MYB; Blue light; TRANSCRIPTION FACTORS; FLAVONOID BIOSYNTHESIS; GENE-EXPRESSION; LOW-TEMPERATURE; ACCUMULATION; ARABIDOPSIS; PROTEIN; HY5; COLORATION; MDMYB1;

D O I：

10.1007/s11103-018-0802-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pp4ERF24 and Pp12ERF96 fine tune blue light-induced anthocyanin biosynthesis via interacting with PpMYB114 and promoting the interaction between PpMYB114 and PpbHLH3, which enhances the expression of PpMYB114-induced PpUFGT. The red coloration of pear fruit is attributed to anthocyanin accumulation, which is transcriptionally regulated by the MYB-bHLH-WD40 complex. A number of ethylene response factors (ERF) have been identified to regulate anthocyanin biosynthesis in different plants. In pear, several ERF transcription factor genes were identified to be potentially involved in the light-induced anthocyanin biosynthesis according to transcriptome data. But the molecular mechanism of these ERFs underlying the regulation of anthocyanin accumulation is unknown. In this study, exposure of 'Red Zaosu' pear, a mutant of 'Zaosu' pear, to blue light significantly induced the anthocyanin accumulation by increasing the expression levels of anthocyanin biosynthetic genes. Gene expression analysis confirmed that the expression of Pp4ERF24 and Pp12ERF96 genes were up-regulated in the process of blue light-induced anthocyanin biosynthesis. Yeast two-hybrid and bimolecular fluorescence complementation assay revealed that Pp4ERF24 and Pp12ERF96 interacted with PpMYB114, but not with PpMYB10. Bimolecular fluorescence complementation assay demonstrated that the interaction between these two ERFs and PpMYB114 enhanced the interaction between PpMYB114 and PpbHLH3. Further analysis by dual luciferase assay verified that these two ERFs increased the up-regulation of PpMYB114-mediated PpUFGT expression. Furthermore, co-transformation of Pp12ERF96 with PpMYB114 and PpbHLH3 in tobacco leaves led to enhanced anthocyanin accumulation. Transient overexpression of Pp4ERF24 or Pp12ERF96 alone in 'Red Zaosu' pear fruit also induced anthocyanin biosynthesis in pear peel. Our findings provide insights into a mechanism involving the synergistic interaction of ERFs with PpMYB114 to regulate light-dependent coloration and anthocyanin biosynthesis in pear fruits.

引用

页码：67 / 78

页数：12

共 1 条

[1] Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in ‘Red Zaosu’ pear fruits by interacting with MYB114
Junbei Ni
Songling Bai
Yuan Zhao
Minjie Qian
Ruiyan Tao
Lei Yin
Ling Gao
Yuanwen Teng
Plant Molecular Biology, 2019, 99 : 67 - 78

← 1 →