Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

被引：3
作者：
Ignacimuthu, S. [1 ]
Raveendar, S. [1 ]
机构：
[1] Loyola Coll, Entomol Res Inst, Plant Biotechnol Unit, Madras 600034, Tamil Nadu, India
来源：
EUPHYTICA | 2011年 / 179卷 / 02期
关键词：
Rice (Oryza sativa); Agrobacterium transformation; Cry1C; hpt; Gene transfer; Transgenic plants; Insect resistance; THURINGIENSIS DELTA-ENDOTOXIN; YELLOW STEM BORER; BACILLUS-THURINGIENSIS; TRANSGENIC RICE; HIGHLY RESISTANT; CRY1AB GENE; PLANTS; TUMEFACIENS; EXPRESSION; TOBACCO;
D O I：
10.1007/s10681-010-0308-7
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The rice leaffolder (RLF), Cnaphalocrocis medinalis is an important pest of rice that causes severe damage in many areas of the world. The plants were transformed with fully modified (plant codon optimized) synthetic Cry1C coding sequences as well as with the hpt and gus genes, coding for hygromycin phosphotransferase and beta-glucuronidase, respectively. Cry1C sequences placed under the control of doubled 35S promoter plus the AMV leader sequence, and hpt and gus genes driven by cauliflower mosaic virus 35S promoter, were used in this study. Embryogenic calli after cocultivation with Agrobacterium were selected on the medium containing hygromycin B. A total of 67 hygromycin-resistant plants were regenerated. PCR and Southern blot analyses of primary transformants revealed the stable integration of Cry1C coding sequences into the rice genome with predominant single copy integration. R(1) progeny plants disclosed a monogenic pattern (3:1) of transgene segregation as confirmed by molecular analyses. These transgenic lines were highly resistant to rice leaffolder (RLF), Cnaphalocrocis medinalis as revealed by insect bioassay.
引用
收藏
页码：277 / 286
页数：10
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