Synthesis, characterization and biological safety of O-carboxymethyl chitosan used to treat Sarcoma 180 tumor

被引：118

作者：

Zheng, Meiling ^{[1
]}

Han, Baoqin ^{[1
]}

Yang, Yan ^{[1
]}

Liu, Wanshun ^{[1
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 86卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

Carboxymethyl chitosan; Sarcoma; 180; Tumor; Biological safety; Biomaterial; IN-VITRO; SKIN IRRITATION; DRUG-DELIVERY; CHITIN; FIBROBLASTS; PROLIFERATION; DERIVATIVES; COMPOSITE; VIVO;

D O I：

10.1016/j.carbpol.2011.04.038

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Carboxymethyl chitosan (CM-chitosan) was prepared and characterized by FTIR and NMR spectroscopy, and its biological safety in tumor application was investigated both in vitro and in vivo. Cytotoxicity studies by MTT assay indicated that CM-chitosan was safe both on normal cell L02 and three tumor cell lines: Bel-7402, SGC-7901 and Hela in vitro. CM-chitosan also improved the TGF-alpha secretion of L02 cell (P < 0.05), whereas decreased levels of TGF-alpha and VEGF secreted by Bel-7402 cell (P < 0.05), which are compatible with the observations at cell levels. In vivo, transplant tumor model of sarcoma 180 was established in mice and CM-chitosan was administered through intraperitoneal injection. Experimental data indicated that CM-chitosan was safe in vivo and slightly inhibited growth of sarcoma 180 and enhanced body immunity via elevation of serum IL-2 and TNF-alpha levels in treated mice (P < 0.05). These results suggest that CM-chitosan is safe in tumor application as biomedical material. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：231 / 238

页数：8

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