Structural requirements for nuclear localization of GCMa/Gcm-1

被引：7
作者：
Hashemolhosseini, S
Kilian, K
Kardash, E
Lischka, P
Stamminger, T
Wegner, M
机构：
[1] Univ Erlangen Nurnberg, Inst Biochem, D-91054 Erlangen, Germany
[2] Univ Erlangen Nurnberg, Inst Klin & Mol Virol, D-91054 Erlangen, Germany
来源：
FEBS LETTERS | 2003年 / 553卷 / 03期
关键词：
nuclear localization sequence; nuclear export signal; nuclear import; nucleocytoplasmic shuttling; glial cells missing;
D O I：
10.1016/S0014-5793(03)01037-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
GCM proteins constitute a small transcription factor family. Nuclear localization of Drosophila GCM is mediated by a typical bipartite nuclear localization sequence (NLS) close to the DNA-binding GCM domain. Here, we have analyzed nuclear localization of the mammalian GCM proteins. Whereas GCMb/Gcm-2 contained a classical bipartite NLS, nuclear localization of GCMa/Gcm-1 was mediated by two regions without resemblance to known NLS, one corresponding to the amino-terminal part of the GCM domain, the second defined as a tyrosine-and-proline-rich carboxy-terminal region. Nuclear import was counteracted by an amino-terminal nuclear export activity. This complex regulation of subcellular localization has important implications for GCMa/Gcm-1 function. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：315 / 320
页数：6
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