Tilia tomentosa pectins exhibit dual mode of action on phagocytes as β-glucuronic acid monomers are abundant in their rhamnogalacturonans I

被引：35

作者：

Georgiev, Yordan N. ^{[1
,2
]}

Paulsen, Berit S. ^{[3
]}

Kiyohara, Hiroaki ^{[4
]}

Ciz, Milan ^{[5
]}

Ognyanov, Manol H. ^{[1
,2
]}

Vasicek, Ondrej ^{[5
,9
]}

Rise, Frode ^{[6
]}

Denev, Petko N. ^{[1
,2
]}

Lojek, Antonin ^{[5
]}

Batsalova, Tsvetelina G. ^{[7
]}

Dzhambazov, Balik M. ^{[7
]}

Yamada, Haruki ^{[4
]}

Lund, Reidar ^{[6
]}

Barsett, Hilde ^{[3
]}

Krastanov, Albert I. ^{[8
]}

Yanakieva, Irina Z. ^{[1
,2
]}

Kratchanova, Maria G. ^{[1
,2
]}

机构：

[1] Bulgarian Acad Sci, Ctr Phytochem, Inst Organ Chem, Lab Biol Act Subst, 139 Ruski Blvd, BG-4000 Plovdiv, Bulgaria

[2] Innovat Technol Ctr Ltd, 20 Dr GM Dimitrov Str, BG-4000 Plovdiv, Bulgaria

[3] Univ Oslo, Sch Pharm, Dept Pharmaceut Chem, POB 1068, NO-0316 Oslo, Norway

[4] Kitasato Univ, Kitasato Inst Life Sci, Dept Drug Discovery Sci, Minato Ku, 5-9-1 Shirokane, Tokyo 1088641, Japan

[5] Czech Acad Sci, Inst Biophys, Dept Free Radical Pathophysiol, 135 Kralovopolska, CZ-61265 Brno, Czech Republic

[6] Univ Oslo, Dept Chem, POB 1033, NO-0315 Oslo, Norway

[7] Plovdiv Univ Paisii Hilendarski, Dept Dev Biol, 24 Tsar Assen Str, BG-4000 Plovdiv, Bulgaria

[8] Univ Food Technol, Dept Biotechnol, 26 Maritza Blvd, BG-4002 Plovdiv, Bulgaria

[9] St Annes Univ Hosp Brno, Ctr Biomol & Cellular Engn, Int Clin Res Ctr, 53 Pekarska, CZ-65691 Brno, Czech Republic

来源：

CARBOHYDRATE POLYMERS | 2017年 / 175卷

关键词：

Tilia flowers; Pectin; Immunomodulating polysaccharides; Anti-inflammatory activity; Neutrophils; Peyer's patches; BIOPHYTUM-PETERSIANUM KLOTZSCH; NITRIC-OXIDE PRODUCTION; SIGNALING PATHWAYS; STRUCTURAL CHARACTERISTICS; COLORIMETRIC METHOD; PLANT MUCILAGES; CITRUS PECTIN; URONIC-ACID; SIDE-CHAINS; POLYSACCHARIDES;

D O I：

10.1016/j.carbpol.2017.07.073

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Silver linden flowers contain different pectins (PSI-PSIII) with immunomodulating properties. PSI is a low-esterified pectic polysaccharide with predominant homogalacturonan region, followed by rhamnogalacturonan I (RGI) with arabinogalactan II and RGII (traces) domains. PSII and PSIII are unusual glucuronidated RGI polymers. PSIII is a unique high molecular weight RGI, having almost completely 0-3 glucuronidated GalA units with >30% 0-3 acetylation at the Rha units. Linden pectins induced reactive oxygen species (ROS) and NO generation from non-stimulated whole blood phagocytes and macrophages, resp., but suppressed OZP-(opsonized zymosan particles)-activated ROS generation, LPS-induced iNOS expression and NO production. This dual mode of action suggests their anti-inflammatory activity, which is known for silver linden extracts. PSI expressed the highest complement fixation and macrophage stimulating activities and was active on intestinal Peyer's patch cells. PSIII was active on non-stimulated neutrophils, as it induced beta(2)-integrin expression, revealing that acetylated and highly glucuronidated RGI exhibits immunomodulating properties via phagocytes. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：178 / 191

页数：14

共 82 条

[21] Neuroprotective effects of ginseng pectin through the activation of ERK/MAPK and Akt survival signaling pathways [J].