Implication of metabolomic profiles to wide thermoneutral zone in Mongolian gerbils (Meriones unguiculatus)

被引:6
作者
Shi, Yaolong [1 ,2 ]
Wang, Dehua [1 ]
机构
[1] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China
[2] Univ Chinese Acad Sci, Beijing, Peoples R China
来源
INTEGRATIVE ZOOLOGY | 2016年 / 11卷 / 04期
关键词
basal metabolic rate; gas chromatography-mass spectrometry metabolomics; low molecular weight antioxidants; oxidative stress; thermoneutral zone; OXIDATIVE STRESS; HEAT-SHOCK; DAMAGED PROTEINS; BODY-MASS; ACID; MICE; MAMMALS; DEGRADATION; METHIONINE; CELLS;
D O I
10.1111/1749-4877.12179
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Mongolian gerbils (Meriones unguiculatus) have evolved a wide thermoneutral zone (26.5-38.9 degrees C) and high upper critical temperature, and appear to have a high tolerance for heat exposure. Here, we use a metabolomic approach to measure global metabolite profiles for gerbils between lower (27 degrees C) and upper critical temperatures (38 degrees C) to investigate the role of metabolomic characterization in maintaining basal metabolic rates within a wide thermoneutral zone. We found that in serum and liver, 14 and 19 metabolites were significantly altered, respectively. In the aerobic respiration-related tricarboxylic cycle (TCA), 5 intermediates (isocitric acid, cis-aconitic acid, -ketoglutaric acid, fumaric acid and malic acid) were increased in serum in 38 degrees C animals; however, no such increase was found in the liver. A stable level of hepatic TCA cycle intermediates may be related to the steady state of aerobic respiration at 38 degrees C. Metabolomic results also revealed that acute heat exposure caused increased oxidative stress and low molecular weight antioxidants in Mongolian gerbils. Increased methionine and 2-hydroxybutyrate suggest an accelerated synthesis of glutathione. Increased urate and its precursors, inosine and hypoxanthine, were detected at 38 degrees C. Glucuronate, threonate and oxalate involved in ascorbate synthesis and degradation were increased in serum at 38 degrees C. In conclusion, although dramatic metabolomic variation was found, a stable hepatic TCA cycle may contribute to maintaining a constant basal metabolic rate within a wide thermoneutral zone in Mongolian gerbils.
引用
收藏
页码:282 / 294
页数:13
相关论文
共 69 条
  • [61] OXIDATION OF METHIONYL RESIDUES IN PROTEINS - TOOLS, TARGETS, AND REVERSAL
    VOGT, W
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (01) : 93 - 105
  • [62] Metabolism and thermoregulation in the Mongolian gerbil Meriones unguiculatus
    Wang, DH
    Wang, YS
    Wang, ZW
    [J]. ACTA THERIOLOGICA, 2000, 45 (02): : 183 - 192
  • [63] Metabolomics of aerobic metabolism in mice selected for increased maximal metabolic rate
    Wone, Bernard
    Donovan, Edward R.
    Hayes, Jack P.
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2011, 6 (04): : 399 - 405
  • [64] Oxidative stress in response to natural and experimentally elevated reproductive effort is tissue dependent
    Xu, Yan-Chao
    Yang, Deng-Bao
    Speakman, John R.
    Wang, De-Hua
    [J]. FUNCTIONAL ECOLOGY, 2014, 28 (02) : 402 - 410
  • [65] Amine Metabolomics of Hyperglycemic Endothelial Cells using Capillary LC-MS with Isobaric Tagging
    Yuan, Wei
    Zhang, Junxiang
    Li, Shuwei
    Edwards, James L.
    [J]. JOURNAL OF PROTEOME RESEARCH, 2011, 10 (11) : 5242 - 5250
  • [66] C. elegans metallothioneins: response to and defence against ROS toxicity
    Zeitoun-Ghandour, Sukaina
    Leszczyszyn, Oksana I.
    Blindauer, Claudia A.
    Geier, Florian M.
    Bundy, Jacob G.
    Stuerzenbaum, Stephen R.
    [J]. MOLECULAR BIOSYSTEMS, 2011, 7 (08) : 2397 - 2406
  • [67] Zhang Z, 1998, ECOLOGY MANAGEMENT R
  • [68] Seasonal changes in thermogenesis and body mass in wild Mongolian gerbils (Meriones unguiculatus)
    Zhang, Zhi-Qiang
    Wang, De-Hua
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2007, 148 (02): : 346 - 353
  • [69] Zhou Li-Zhi, 2001, Acta Zoologica Sinica, V47, P616