Methionine enkephalin inhibits influenza A virus infection through upregulating antiviral state in RAW264.7 cells

被引：10

作者：

Tian, Jing ^{[1
,2
]}

Qu, Na ^{[3
]}

Jiao, Xue ^{[4
]}

Wang, Xiaonan ^{[2
]}

Geng, Jin ^{[5
]}

Griffin, Noreen ^{[6
]}

Shan, Fengping ^{[2
]}

机构：

[1] Jinzhou Med Univ, Sch Basic Med Sci, Dept Immunol, Jinzhou 121001, Peoples R China

[2] China Med Univ, Sch Basic Med Sci, Dept Immunol, Shenyang 110122, Peoples R China

[3] China Med Univ, Canc Hosp, Dept Gynecol, Shenyang 110042, Peoples R China

[4] China Med Univ, Dept Translat Med, Teaching Hosp 4, Shenyang 110032, Peoples R China

[5] China Med Univ, Dept Ophthalmol, Teaching Hosp 1, Shenyang 110001, Peoples R China

[6] Immune Therapeut Inc, 37 North Orange Ave,Suite 607, Orlando, FL 32801 USA

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2020年 / 78卷

关键词：

Methionine enkephalin; Influenza A virus; RAW264.7; cell; Opioid receptor; Toll-like receptors; Antiviral effect; NF-KAPPA-B; INDUCED INFLAMMATION; OPIOID RECEPTORS; MACROPHAGES; IMMUNOMODULATION; TRANSMISSION; PATHOGENESIS; PROTECTS; CANCER;

D O I：

10.1016/j.intimp.2019.106032

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

MENK, as an immune adjuvant, has potential immune-regulatory activity on innate and adaptive immune cells. The aim of this work was to investigate the antiviral effect of MENK on influenza virus-infected murine macrophage cells (RAW264.7) and its underlying mechanisms. The results showed that MENK markedly inhibited influenza A virus (H1N1) replication in pre- and post-MENK treatment, especially in pre-MENK treatment. The mechanisms exploration revealed that MENK (10 mg/mL) significantly inhibited the nucleoprotein (NP) of influenza virus and up-regulated levels of IL-6, TNF-alpha and IFN-beta compared with those in H1N1 control group. Further experiments confirmed that antiviral effects of MENK was associated with promotion of opioid receptor (MOR) as well as activation of NF-kappa B p65 inducing cellular antiviral status. The data suggest that MENK should be potential candidate for prophylactic or therapeutic treatment against H1N1 influenza virus.

引用

页数：13

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