Specific subcellular localization of siRNAs delivered by lipoplex in MCF-7 breast cancer cells

In order to better understand the mechanism of delivery of siRNAs by lipid-based vectors, we investigated the subcellular distribution of siRNAs directed against cyclin D1 delivered by the DLS system in the breast cancer cell line MCF-7. Cells were treated with cyclopentenone or 17 beta-estradiol to modulate the level of expression of cyclin D1 mRNA. We qualitatively observed that siRNA localized to specific cytoplasmic compartments in the periphery of the nucleus in granular-like structures that do not correspond to early endosomal vesicles. In cells treated with either cyclopentenone or 17 beta-estradiol cellular distribution of siRNAs was not affected but variations in the amount of siRNAs present in cells were found. Ale suggest these variations might be associated with the effects of cyclopentenone and 17 beta-estradiol in cyclin D1 gene expression. Low cytotoxicity and highly cellular uptake of lipoplexes was observed in the presence of serum indicating that the DLS system could be a useful tool for siRNA vectorization in vitro and in vivo. (c) 2007 Elsevier Masson SAS. All fights reserved.