New dimensions for VIGS in plant functional genomics

被引：234

作者：

Senthil-Kumar, Muthappa ^{[1
]}

Mysore, Kirankumar S. ^{[1
]}

机构：

[1] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA

来源：

TRENDS IN PLANT SCIENCE | 2011年 / 16卷 / 12期

基金：

美国国家科学基金会;

关键词：

STRIPE-MOSAIC-VIRUS; SMALL-INTERFERING RNAS; TOBACCO-RATTLE-VIRUS; NICOTIANA-BENTHAMIANA; ARABIDOPSIS-THALIANA; DNA METHYLATION; GENE-EXPRESSION; SWEET-POTATO; TOMATO; BIOSYNTHESIS;

D O I：

10.1016/j.tplants.2011.08.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Virus-induced gene silencing (VIGS) is an efficient tool for gene function studies. It has been used to perform both forward and reverse genetics to identify plant genes involved in several plant processes. However, this technology has not yet been used to its full potential. This can be attributed to several of its limitations such as inability to silence genes during seed germination and the non-stable nature of silencing. However, several recent studies have shown that these limitations can now be overcome. In this review, we will discuss the limitations of VIGS and suitable solutions. In addition, we also describe the recent improvements and future prospects of using VIGS in plant biology.

引用

页码：656 / 665

页数：10

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