Long-term in vivo imaging of human angiogenesis:: Critical role of bone marrow-derived mesenchymal stem cells for the generation of durable blood vessels

被引：64

作者：

Sanz, Laura ^{[1
]}

Santos-Valle, Patricia ^{[1
]}

Alonso-Camino, Vanesa ^{[1
]}

Salas, Clara ^{[2
]}

Serrano, Antonio ^{[3
]}

Vicario, Jose Luis ^{[4
]}

Cuesta, Angel M. ^{[1
]}

Compte, Marta ^{[1
]}

Sanchez-Martin, David ^{[1
]}

Alvarez-Vallina, Luis ^{[1
]}

机构：

[1] Hosp Univ Puerta Hierro, Unidad Inmunol Mol, Madrid 28035, Spain

[2] Hosp Univ Puerta Hierro, Dept Patol, Madrid 28035, Spain

[3] Hosp Univ Doce Octubre, Dept Inmunol, Madrid 28041, Spain

[4] Ctr Transfus, Dept Histocompatibilidad, Madrid 28032, Spain

来源：

MICROVASCULAR RESEARCH | 2008年 / 75卷 / 03期

关键词：

angiogenesis; bioluminescence imaging; tissue engineering; long-lasting blood vessels;

D O I：

10.1016/j.mvr.2007.11.007

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Angiogenesis is a multistep process that encompasses complex molecular and cellular interactions that can not be recapitulated in vitro. Here, we demonstrate that vasculature generated from lentivirally transduced human primary endothelial cells expressing firefly luciferase and co-implanted with human bone marrow mesenchymal stem cells in immunodeficient mice can be assessed quantitatively by in vivo whole body bioluminescence imaging for more than 120 days. Luciferase activity correlated with the formation of a network of functional, mature blood vessels of human nature inside the implant that critically depend on the presence of mesenchymal stem cells. In summary, our study offers an unprecedented opportunity to perform long-term serial analysis of the molecular events involved in the angiogenic process and monitoring responses to anti-angiogenic agents. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：308 / 314

页数：7

共 23 条

[1] Benefits of targeting both pericytes and endothelial cells in the tumor vasculature with kinase inhibitors [J].

Bergers, G ;

Song, S ;

Meyer-Morse, N ;

Bergsland, E ;

Hanahan, D .

JOURNAL OF CLINICAL INVESTIGATION, 2003, 111 (09) :1287-1295

[2] Angiogenesis in life, disease and medicine [J].

Carmeliet, P .

NATURE, 2005, 438 (7070) :932-936

[3] Inhibition of tumor growth in vivo by in situ secretion of bispecific anti-CEA x anti-CD3 diabodies from lentivirally transduced human lymphocytes [J].

Compte, M. ;

Blanco, B. ;

Serrano, F. ;

Cuesta, A. M. ;

Sanz, L. ;

Bernad, A. ;

Holliger, P. ;

Alvarez-Vallina, L. .

CANCER GENE THERAPY, 2007, 14 (04) :380-388

[4] Molecular imaging of tumor angiogenesis [J].

Costouros, NG ;

Diehn, FE ;

Libutti, SK .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2002, :72-78

[5] Imaging angiogenesis [J].

Dobrucki, Lawrence W. ;

Sinusas, Albert J. .

CURRENT OPINION IN BIOTECHNOLOGY, 2007, 18 (01) :90-96

[6] Quantification of human angiogenesis in immunodeficient mice using a photon counting-based method [J].

Dong, Zhihong ;

Neiva, Kathleen G. ;

Jin, Taocong ;

Zhang, Zhaocheng ;

Hall, Daniel E. ;

Mooney, David J. ;

Polverini, Peter J. ;

Noer, Jacques E. .

BIOTECHNIQUES, 2007, 43 (01) :73-77

[7] Induction, differentiation, and remodeling of blood vessels after transplantation of Bcl-2-transduced endothelial cells [J].

Enis, DR ;

Shepherd, BR ;

Wang, YN ;

Qasim, A ;

Shanahan, CM ;

Weissberg, PL ;

Kashgarian, M ;

Pober, JS ;

Schechner, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (02) :425-430

[8] Combined inhibition of VEGF- and PDGF-signaling enforces tumor vessel regression by interfering with pericyte-mediated endothelial cell survival mechanisms [J].

Erber, R ;

Thurnher, A ;

Katsen, AD ;

Groth, G ;

Kerger, H ;

Hammes, HP ;

Menger, MD ;

Ullrich, A ;

Vajkoczy, P .

FASEB JOURNAL, 2003, 17 (15) :338-340

[9] Angiogenesis as a therapeutic target [J].

Ferrara, N ;

Kerbel, RS .

NATURE, 2005, 438 (7070) :967-974

[10]

Fong TAT, 1999, CANCER RES, V59, P99

← 1 2 3 →