The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor

被引：135

作者：

Cobine, P

Wickramasinghe, WA

Harrison, MD

Weber, T

Solioz, M

Dameron, CT

机构：

[1] Univ Queensland, Natl Res Ctr Environm Toxicol, Coopers Plains, Qld 4108, Australia

[2] Univ Bern, Dept Clin Pharmacol, CH-3010 Bern, Switzerland

来源：

FEBS LETTERS | 1999年 / 445卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

copper chaperone; Enterococcus hirae; metalloregulation; metal transfer; repressor;

D O I：

10.1016/S0014-5793(99)00091-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the cop operon which effects copper homeostasis in Enterococcus hirae is controlled by the copper responsive repressor CopY, Purified Zn(II)CopY binds to a synthetic cop promoter fragment in vitro. Here we show that the 8 kDa protein CopZ acts as a copper chaperone by specifically delivering copper(I) to Zn(II)CopY and releasing CopY from the DNA. As shown by gel filtration and luminescence spectroscopy, two copper(I) are thereby quantitatively transferred from Cu(I)CopZ to Zn(II)CopY, with displacement of the zinc(YI) and transfer of copper from a non-luminescent, exposed, binding site in CopZ to a luminescent, solvent shielded, binding site in CopY, (C) 1999 Federation of European Biochemical Societies.

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页码：27 / 30

页数：4

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