Angiopoietin-1 protects neurons by inhibiting autophagy after neuronal oxygen-glucose deprivation/recovery injury

被引:9
|
作者
Yin, Zhaoyang [1 ]
Gong, Ge [2 ]
Zhu, Chao [3 ]
Wang, Bin [3 ]
Sun, Chao [3 ]
Liu, Xinhui [3 ]
Yin, Jian [3 ]
机构
[1] Xuzhou Med Univ, Peoples Hosp Lianyungang 1, Dept Orthoped, Affiliated Lianyungang Hosp, Lianyungang, Peoples R China
[2] Nanjing Univ, Jinling Hosp, Dept Geriatr, Med Sch, Nanjing, Peoples R China
[3] Nanjing Med Univ, Dept Orthoped, Affiliated Jiangning Hosp, Nanjing, Peoples R China
关键词
angiopoietin-1; autophagy; neurons; oxygen-glucose deprivation; recovery; SPINAL-CORD-INJURY; CHALLENGES; ACTIVATION; APOPTOSIS; RAPAMYCIN;
D O I
10.1097/WNR.0000000000001491
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Angiopoietin-1 (Ang-1) is a new neuroprotective agent, which can protect neurons from apoptosis. Increased autophagy in neurons subjected to oxygen-glucose deprivation/recovery (OGD/R) injury may lead to autophagic cell death; therefore, the present study investigated the effect of Ang-1 on neurons subjected to OGD/R injury. Neuronal viability was detected by using the Cell Counting Kit-8, which was then used to select the appropriate concentration of Ang-1 and rapamycin used in the OGD/R injury model. The mechanistic role of Ang-1 was observed by detecting the survival rate of neurons and the level of autophagy. Results showed that Ang-1 significantly reduced neuronal cell injury induced by OGD/R and the expression of the autophagy-related proteins LC3 II/I and Beclin-1, and increased the expression of P62/SQSTM1. However, the neuroprotective effects of Ang-1 were counteracted by rapamycin, an autophagy activating agent. The changes of autophagy intensity were further confirmed by transmission electron microscopy observation of autophagosomes. Ang-1 appears to have a neuroprotective role by inhibiting autophagy expression in OGD/R. Thus, these findings could be useful for the treatment of OGD/R injury.
引用
收藏
页码:825 / 832
页数:8
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