Highly efficient enrichment of phosphopeptides by a magnetic lanthanide metal-organic framework

被引：56
作者：
Xie, Yiqin
Deng, Chunhui [1 ]
机构：
[1] Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Dept Chem, Shanghai 200433, Peoples R China
来源：
TALANTA | 2016年 / 159卷
基金：
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词：
MOF; Phosphopeptide enrichment; Lanthanide; Mass spectrometry; SELECTIVE ENRICHMENT; MASS-SPECTROMETRY; QUANTITATIVE PHOSPHOPROTEOMICS; TI4+ IMMOBILIZATION; FACILE PREPARATION; NANOPARTICLES; PEPTIDES; PHOSPHORYLATION; SEPARATION; PROTEINS;
D O I：
10.1016/j.talanta.2016.05.075
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Highly efficient enrichment of phosphopeptides from complex biological samples is crucial prior to mass spectrometry analysis due to the low abundance and ion suppression effects. In this study, a facile route was designed for preparation of a magnetic erbium(Er)-based metal-organic framework (denoted as Fe3O4@PDA@Er(btc)), which was synthesized with 1,3,5-benzenetricarboxylic acid(H(3)btc) as ligand and grafted on the polydopamine (PDA) - coated Fe3O4. The as-prepared material exhibited ultra-high sensitivity (detection limit of 20 amol/mu L) and selectivity at a low mass ratio of beta-Casein/BSA (1:500). Moreover, it was also investigated for enrichment of phosphopeptides from human serum, which provided a promising technique for highly efficient enrichment of low-abundance phosphorylated peptides in the practical application. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：1 / 6
页数：6
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