TGF-β1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

被引：146
作者：
Liu, Ying [1 ]
Li, Yue [2 ]
Li, Ning [1 ]
Teng, Wen [1 ]
Wang, Min [1 ]
Zhang, Yingbo [1 ]
Xiao, Zhibo [1 ]
机构：
[1] Harbin Med Univ, Affiliated Hosp 2, Dept Plast & Aesthet Surg, Harbin 150081, Peoples R China
[2] Harbin Med Univ, Affiliated Hosp 2, Dept Gen Surg, Harbin 150081, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金;
关键词：
HYPERTROPHIC SCAR; MESSENGER-RNA; IN-VITRO; NORMAL SKIN; EXPRESSION; PROTEIN; INVASION; MIR-21; PTEN; PHOSPHATASE;
D O I：
10.1038/srep32231
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
TGF-beta 1, upregulated in keloid tissue, promotes the proliferation, collagen formation and differentiation of dermal fibroblasts. miR-21 is one of microRNAs first found in human genome. The aim of our study is to explore the mechanisms of miR-21 in TGF-beta 1-induced scar fibroblasts proliferation and transdifferentiation. In the present study, first we found that TGF-beta 1 promoted scar fibroblasts proliferation and transdifferentiation via up-regulating miR-21 expression, which could be attenuated when miR-21 was inhibited. Overexpression of miR-21 had similar effect as TGF-beta 1 on proliferation and transdifferentiation. Additionally, TGF-beta 1 increased the expressions and activities of MMP2 and MMP9 in keloid fibroblasts, which was suppressed by miR-21 inhibition. Finally, the results demonstrated that PTEN/AKT signaling pathway played important role in TGF-beta 1-induced transdifferentiation. In conclusion, our study suggests that TGF-beta 1 promotes keloid fibroblasts proliferation and transdifferentiation via up-regulation of miR-21 and PTEN/AKT signalling pathway plays important role in this process, which provides a potential theoretical basis for clinical treatment of skin scars.
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页数：9
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