TGF-β1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

被引：138

作者：

Liu, Ying ^{[1
]}

Li, Yue ^{[2
]}

Li, Ning ^{[1
]}

Teng, Wen ^{[1
]}

Wang, Min ^{[1
]}

Zhang, Yingbo ^{[1
]}

Xiao, Zhibo ^{[1
]}

机构：

[1] Harbin Med Univ, Affiliated Hosp 2, Dept Plast & Aesthet Surg, Harbin 150081, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 2, Dept Gen Surg, Harbin 150081, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

HYPERTROPHIC SCAR; MESSENGER-RNA; IN-VITRO; NORMAL SKIN; EXPRESSION; PROTEIN; INVASION; MIR-21; PTEN; PHOSPHATASE;

D O I：

10.1038/srep32231

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

TGF-beta 1, upregulated in keloid tissue, promotes the proliferation, collagen formation and differentiation of dermal fibroblasts. miR-21 is one of microRNAs first found in human genome. The aim of our study is to explore the mechanisms of miR-21 in TGF-beta 1-induced scar fibroblasts proliferation and transdifferentiation. In the present study, first we found that TGF-beta 1 promoted scar fibroblasts proliferation and transdifferentiation via up-regulating miR-21 expression, which could be attenuated when miR-21 was inhibited. Overexpression of miR-21 had similar effect as TGF-beta 1 on proliferation and transdifferentiation. Additionally, TGF-beta 1 increased the expressions and activities of MMP2 and MMP9 in keloid fibroblasts, which was suppressed by miR-21 inhibition. Finally, the results demonstrated that PTEN/AKT signaling pathway played important role in TGF-beta 1-induced transdifferentiation. In conclusion, our study suggests that TGF-beta 1 promotes keloid fibroblasts proliferation and transdifferentiation via up-regulation of miR-21 and PTEN/AKT signalling pathway plays important role in this process, which provides a potential theoretical basis for clinical treatment of skin scars.

引用

页数：9

共 35 条

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